Protein Info for LRK55_RS09895 in Rhodanobacter denitrificans MT42

Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 transmembrane" amino acids 243 to 253 (11 residues), see Phobius details amino acids 269 to 286 (18 residues), see Phobius details TIGR00858: 8-amino-7-oxononanoate synthase" amino acids 23 to 384 (362 residues), 465.9 bits, see alignment E=3.6e-144 PF00155: Aminotran_1_2" amino acids 45 to 384 (340 residues), 192.1 bits, see alignment E=8.9e-61

Best Hits

Swiss-Prot: 58% identical to BIOF_ALKEH: 8-amino-7-oxononanoate synthase (bioF) from Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 56% identity to nhl:Nhal_0765)

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (401 amino acids)

>LRK55_RS09895 8-amino-7-oxononanoate synthase (Rhodanobacter denitrificans MT42)
MPRPDLLERMAVQTAERERAGLLRRLRTIDHADGPWLASGSRCLLAFCSNDYLGLAQHPQ
LVAALTRAAREEGVGSTSAHLICGHRSAHAELEEALAAWTGRERALLFSTGYMANLGVLQ
ALLARGDVCVQDKLNHACLLDGAKLSGAELKRYPHADVAAAARQLAANPGAAALLATDGV
FSMDGDVAPLRALVQLCAGERATLMVDDAHGLGVLGDDGAGSLSAAKLGQHEVPLLMATL
GKALGCSGAFVAGPAALIEGLVQFARPYIYTTAMPPALAAAALEAVRLAQSETWRRDKLS
ALIGRFRRGAAELGLPLAASPSAIQPLLLGGAEAALGAAQALERQGLLVTAIRPPTVPAG
QARLRITLSAAHEEIHVDRLLGALETLRLTAPCKAPPGDHV