Protein Info for LRK55_RS08750 in Rhodanobacter denitrificans MT42

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 TIGR00234: tyrosine--tRNA ligase" amino acids 7 to 399 (393 residues), 376.1 bits, see alignment E=1.2e-116 PF00579: tRNA-synt_1b" amino acids 30 to 314 (285 residues), 264.7 bits, see alignment E=5.3e-83

Best Hits

Swiss-Prot: 80% identical to SYY_XANC5: Tyrosine--tRNA ligase (tyrS) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 83% identity to psu:Psesu_0168)

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>LRK55_RS08750 tyrosine--tRNA ligase (Rhodanobacter denitrificans MT42)
MSELERALATMARGADEIIKREDLAERLKQGRPLRIKAGFDPTAPDLHLGHTVLLNKMRQ
FQDLGHQVIFLIGDFTGMIGDPTGKNVTRKPLSRDDVLANAETYAEQVYKVLDREKTELR
FNSEWFGKMSAADMIRLAAQHTVARMLERDDFTKRYAAQQPIAIHEFLYPLVQGYDSVAL
KADVELGGTDQKFNLLMGRALQEHHGQPPQIVLTMPLLEGLDGVNKMSKSLGNYIGISEP
AIDIVTKTMKIGDELMWRWFELLSFDVSLDELARMRQDIASGALNPRDAKLRLARELATR
FHDAAAAEQAIAGWHAVVRGEGDTSLLPQSDVAVPAEGMRLAALLTAAGLTASNSEANRK
LKERAVRIDADVAEDAQRVFHAGFEGVLQVGKRNFVRVRLVLA