Protein Info for LRK55_RS08450 in Rhodanobacter denitrificans MT42

Annotation: GH1 family beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 69% identity to sal:Sala_1019)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

neolinustatin bioactivation (2/3 steps found)
linamarin degradation (1/2 steps found)
lotaustralin degradation (1/2 steps found)
linustatin bioactivation (2/4 steps found)
cellulose degradation II (fungi) (1/3 steps found)
coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
α-tomatine degradation (1/6 steps found)
firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (453 amino acids)

>LRK55_RS08450 GH1 family beta-glucosidase (Rhodanobacter denitrificans MT42)
MTRPSFRFPDGFHWGAATSAYQIEGSPLADGAGPSIWQRFAHTPGMMVNGDTGDVACDHY
RRYREDVRLMKALGLKGYRFSISWARVLPEGSGRVNPKGLDFYSRLVDELLEHGIAPNAT
LFHWDLPAALDDRGGWLNRDSAYWFAEYAEVMFKALDDRVPRWSTLNEPWVVTDGGYLHG
ALAPGHRSKYEAPLAAHNLMRASGAGIQAYRAHGKHEIGVVFNIEPKYPHSDSAGDLAAT
ARAHAYMNQQFADPALLGSYPPELKEIFGDAWPDFPAEDFKLTKQPVDFVGINYYTRAVV
KHDANAYPLHAVSVRQPNRTYTETGWEVFEQGLTDTLSWFKGRYGDIPLYITENGSAFYD
PPVAEGEVLDDPLRTNYLRKHLQALHRAIAAGVNLKGYYAWSLLDNLEWSLGFSKRFGLY
HVDFATQQRTPKASAKLYAQVIESNGAVLDEDA