Protein Info for LRK55_RS08360 in Rhodanobacter denitrificans MT42

Annotation: shikimate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00507: shikimate dehydrogenase
  • amino acids 6 to 273 (268 residues), 225.4 bits, E=3.7e-71
• PF08501: Shikimate_dh_N
  • amino acids 8 to 90 (83 residues), 80.3 bits, E=1.5e-26
• PF01488: Shikimate_DH
  • amino acids 116 to 195 (80 residues), 33.8 bits, E=5.1e-12
• PF18317: SDH_C
  • amino acids 242 to 272 (31 residues), 33.5 bits, 4.2e-12

Best Hits

Swiss-Prot: 57% identical to AROE_XYLFA: Shikimate dehydrogenase (NADP(+)) (aroE) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K00014, shikimate dehydrogenase [EC: 1.1.1.25] (inferred from 57% identity to xfa:XF0624)

Predicted SEED Role

"Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 1.1.1.25)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of chorismate metabolism (40/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.25

Protein Sequence (276 amino acids)

>LRK55_RS08360 shikimate dehydrogenase (Rhodanobacter denitrificans MT42)
MPVAQFAVFGHPIAHSLSPTIHQAFARQFEIELTYRAIDVEPAGFAAAVRRFFADGGRGA
NVTLPHKAAAFALADERSVAATRAGSANVLAALVAGRLAAHNTDGDGLVRDLTERHGLDL
RGHTALLLGAGGAAHGVAWNLLDAGVATLTIVSRTATSADALADAIGEPARAHTRYWSDL
AGIGSYDLIVNATSAGVLGVSLQLPPSLVGARALCYDLSYGTAASSFLSWATAAGARYAF
DGLGMLVETAADAFALWHGRRPDTEPVYQALRRQSA