Protein Info for LRK55_RS08270 in Rhodanobacter denitrificans MT42

Annotation: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF10396: TrmE_N" amino acids 7 to 120 (114 residues), 128.4 bits, see alignment E=3.2e-41 TIGR00450: tRNA modification GTPase TrmE" amino acids 12 to 447 (436 residues), 312.3 bits, see alignment E=6.3e-97 PF12631: MnmE_helical" amino acids 123 to 444 (322 residues), 187.8 bits, see alignment E=5.7e-59 TIGR00231: small GTP-binding protein domain" amino acids 217 to 335 (119 residues), 76.8 bits, see alignment E=1.7e-25 PF02421: FeoB_N" amino acids 218 to 335 (118 residues), 38.5 bits, see alignment E=1.7e-13 PF01926: MMR_HSR1" amino acids 218 to 307 (90 residues), 79.2 bits, see alignment E=5.3e-26

Best Hits

Swiss-Prot: 54% identical to MNME_SALG2: tRNA modification GTPase MnmE (mnmE) from Salmonella gallinarum (strain 287/91 / NCTC 13346)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 56% identity to eic:NT01EI_3932)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>LRK55_RS08270 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE (Rhodanobacter denitrificans MT42)
MSQSTDTIAAIASAPGAAGVGVLRVSGPAASAIAQALLGRPPQPRHAHFAAFRDGAGELI
DRGLLLHFPAPASYTGEHVLELQGHGSAVLLDALLRRVCELGARLARPGEFTERAFLNGK
LDLAQAEAVADLIAARSQAGARAALQSMEGVFSRKVDALLQSLIALRVHIEAAIDFPEEE
IDFLADPAIAARLERLRAELAELLREAQRGLRLNDGLKVAIVGRPNAGKSSLLNALAGSE
RAIVTPIAGTTRDVLRESLSLDGIALELADTAGLRDTDDEVEREGVRRAHGELQRADVAL
LVTTAADCAADLAFFDALPVGVERIVLINKIDLDQAPARHSQCAGMHWLWASAKTGAGLD
ALREHLKRLAGAGSGEGAFSARRRHVLALQQVGALLDRTAAILTGSRAGELAAEELRQAQ
HALGEITGSYSSDDLLGAIFGSFCIGK