Protein Info for LRK55_RS07515 in Rhodanobacter denitrificans MT42

Annotation: HlyD family efflux transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 transmembrane" amino acids 26 to 47 (22 residues), see Phobius details PF16576: HlyD_D23" amino acids 63 to 294 (232 residues), 52 bits, see alignment E=1.4e-17 PF13533: Biotin_lipoyl_2" amino acids 64 to 112 (49 residues), 45.9 bits, see alignment 9.5e-16 PF13437: HlyD_3" amino acids 220 to 297 (78 residues), 54.2 bits, see alignment E=5.3e-18

Best Hits

Swiss-Prot: 48% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 66% identity to pag:PLES_55501)

MetaCyc: 48% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (397 amino acids)

>LRK55_RS07515 HlyD family efflux transporter periplasmic adaptor subunit (Rhodanobacter denitrificans MT42)
MSSQNPTAPGPAPLAPPARKNPRGPLLRLLAVVVLLAVIGWALWYFLDGRWYEGTDDAYV
NGNVVQITPQVPGTVVSIGADDGDRVHAGDVLVRLDPSDADVALAEAKANLANTVRKVRG
LYSSVSGARADVAARQTAVDKARADYHRRVALARSGAISAEELAHASDALTSAESALTAA
QQQYQTSKVLVDDTVVASHPDVQAASARLRAAFLDDVRATLIAPVDGYVAKRSVQVGQRV
QPGAALMAVVPLHGVWVDANFKETQLTDMRIGQPVTIRSDVYGGAVEYKGKVQSLGVGTG
SAFSLLPAQNATGNWIKIVQRIPVRIVFDDPAQLDQHPLRIGMSLDVDVSLHDRSGPMLS
QRSPSKPAFSTDVYRQQLAKADAAITQIVHANMARTK