Protein Info for LRK55_RS07395 in Rhodanobacter denitrificans MT42

Annotation: glycoside hydrolase family 3 C-terminal domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00933: Glyco_hydro_3
  • amino acids 85 to 336 (252 residues), 122.1 bits, E=5.5e-39
• PF01915: Glyco_hydro_3_C
  • amino acids 371 to 622 (252 residues), 186.4 bits, E=9.2e-59
• PF14310: Fn3-like
  • amino acids 656 to 722 (67 residues), 57.4 bits, E=1.9e-19

Best Hits

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 56% identity to xcc:XCC1404)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

• neolinustatin bioactivation (2/3 steps found)
• linamarin degradation (1/2 steps found)
• lotaustralin degradation (1/2 steps found)
• linustatin bioactivation (2/4 steps found)
• cellulose degradation II (fungi) (1/3 steps found)
• coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
• α-tomatine degradation (1/6 steps found)
• firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Phenylpropanoid biosynthesis
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Protein Sequence (743 amino acids)

>LRK55_RS07395 glycoside hydrolase family 3 C-terminal domain-containing protein (Rhodanobacter denitrificans MT42)
MSRRPRRLSACAFALAVVATATAAGEPPARPWMNASLSPDARAALVVQAMSQDEKFQLIT
TAYGSASDGKPAPACALGSAACAPALSRLGLPALQETDAGLGVARPLNAHSRDVATALPS
GLATAASWDPAVAEAGGAMIGREAHRKGFNVLLAGGVNLLRDPRNGRNFEYAGEDPLLAG
VMAGHAVRGIQSQHVVSTLKHYAVNDLETGRNTMNAKLDRVAARESDLRAFEIALGIGAP
GSVMCAYNRVNGTYACENAWLLDQVLKRDWGYQGFVMSDWGAVHSAAKSALAGLDQESAG
ETFDPQMFFAAPLREAIAKGEVPQARLDDMVRRILRSLFAVGAIDHPAKAAPIDYAADAK
VSQHAAEAGAVLLRNQDNLLPLARTLASVAVIGAHADRGVLAGGGSSAVTAPGGNAVPGL
APTVWPGPVMYHPSAPLAAIARRVGGKASYASGEDIAAAAKLAASSAVAVVFVQQWAAES
FDRPSMALDGHQDALVAAVAKANPRTVVVLENNGPVAMPWLAQTAAVLEAWYPGNAGGEA
IARLLFGEVAPSGRLPLSWPRDASQLPRPAIAGAGLAAIGLPPQGQPADTVDYDIEGADV
GYRWFQRRGSEPLFPFGYGLGYTTFGYGPLTVTTDAAGAVTASFAVTNTGSRAGADVPQL
YVQLPGEQVSRLVGWQKLALRPGETKRVRLALPAVRFARYDDRGHGWRVAAGTYRLLLGR
SSAQIEQRAELRLPAVFPAGAQR