Protein Info for LRK55_RS06875 in Rhodanobacter denitrificans MT42

Annotation: bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1051 PF18327: PRODH" amino acids 15 to 62 (48 residues), 57 bits, see alignment 3.8e-19 PF14850: Pro_dh-DNA_bdg" amino acids 71 to 184 (114 residues), 157.9 bits, see alignment E=2.5e-50 PF01619: Pro_dh" amino acids 194 to 488 (295 residues), 323.9 bits, see alignment E=2.1e-100 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 523 to 1028 (506 residues), 714.4 bits, see alignment E=3.2e-219 PF00171: Aldedh" amino acids 569 to 1028 (460 residues), 374.9 bits, see alignment E=1e-115

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 71% identity to smt:Smal_0308)

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.12

Use Curated BLAST to search for 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1051 amino acids)

>LRK55_RS06875 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA (Rhodanobacter denitrificans MT42)
MTQAILHPELPADGTPARARITAAWLRDETEAVNDLLAQATLPPAEREQVIDVAAGLVTR
VRARAKDQSAVESFMRQYDLSSEEGVLLMCVAEALLRIPDKATADKLIRDKLGEADWKKH
LGQSESLFVNASTWGLMLTGRLVNLAEDTRHDFTGALKRLVGRAGEPAIRLAVRQAMRIM
GHQFVMGRSIDEALDRCAKKEYAVYRYSYDMLGESALTAETAERYQEDYRRAIARIGARG
PFANHTDAPSISVKLSALHPRYEVAKREDARRMLTAKLLELSQLAMQQGIALSVDAEEAD
RLELSLDILGDVFAHPSLEGWNGLGIVVQAYSKRTPFVIDWLVETARAAGRRWYVRLVKG
AYWDAEIKRAQEQGLAGYPLYTRKPNTDVSYLACARKLFDAGVELIYPQFATHNAHSIAA
VHHIARGRPFEFQRLHGMGTDLYAEVIGPQNLNVPCRVYAPVGTHEDLLPYLVRRLLENG
ANTSFVNRVVDETLPVRELVADPCETVRRFASIPHPRIPLPVNLYGELRKNSMGINFSND
NELKALAEAVATKAGPWTAEPLVPGAKGNGPTVPVTNPADRRQAVGSYVSADAALVDQAL
ANAVAAQPGWDRLPAASRAAILEHAAEQLEARRAEFIALCVREAGKSLPDAIAEVREAAD
FLRYYATMARRLFSQPEQLPGPTGESNQLFLNGRGVFVCISPWNFPLAIFLGQVSAALAA
GNSVIAKPAEQTSLIGYAAVKLLHEAGVPLDVLQYLPGDGAVVGAALTRDPRVAGVAFTG
STETAWAINRTLAARNAPIAALVAETGGQNAMIADSSALPEQIVKDVIASAFQSAGQRCS
AARVLFVQEDIADKVIGMLAGAMAELKVGDPGQLSTDVGPVIDEDAKQILVEHAARMDKE
AKKIAEAKLDAEVAAHGTFFAPRAYEIPALATLTREVFGPVLHIVRWKGSELAQVVEQIN
ATGYGLTLGVHSRIDDTVEYIRSHARVGNCYVNRNQIGAVVGVQPFGGESLSGTGPKAGG
PHYLLRFAGERTLTINTTAAGGNASLLTIGE