Protein Info for LRK55_RS06665 in Rhodanobacter denitrificans MT42

Annotation: class I SAM-dependent rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF17785: PUA_3
  • amino acids 19 to 68 (50 residues), 49.4 bits, 1.3e-16
• PF10672: Methyltrans_SAM
  • amino acids 175 to 349 (175 residues), 69.8 bits, E=9.2e-23
• PF01728: FtsJ
  • amino acids 211 to 327 (117 residues), 30.7 bits, E=1.2e-10
• PF03602: Cons_hypoth95
  • amino acids 216 to 317 (102 residues), 24.4 bits, E=8.3e-09
• PF06325: PrmA
  • amino acids 216 to 270 (55 residues), 28.6 bits, 4.1e-10
• PF05175: MTS
  • amino acids 217 to 295 (79 residues), 25.6 bits, E=3.5e-09
• PF13847: Methyltransf_31
  • amino acids 217 to 335 (119 residues), 39.7 bits, E=1.7e-13
• PF13649: Methyltransf_25
  • amino acids 221 to 293 (73 residues), 37.9 bits, E=9.4e-13

Best Hits

Swiss-Prot: 34% identical to RLMI_ENT38: Ribosomal RNA large subunit methyltransferase I (rlmI) from Enterobacter sp. (strain 638)

KEGG orthology group: K06969, ribosomal RNA large subunit methyltransferase I [EC: 2.1.1.-] (inferred from 60% identity to psu:Psesu_3063)

Predicted SEED Role

"LSU m5C1962 methyltransferase RlmI" in subsystem Ribosome biogenesis bacterial

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Protein Sequence (390 amino acids)

>LRK55_RS06665 class I SAM-dependent rRNA methyltransferase (Rhodanobacter denitrificans MT42)
MNTPAALPIVRLKSDRSPGHPWVWSAQVHKPEAKLPPGSLVEAHDARGRFVGRAFWNGHA
RIALRLLDTHPDASIDADWIAARIARAVQLRRDWLQLDAVSDAWRVVHSEGDDLSGLIVD
RYADVLVIEYFAAGMWRFREAIHAALLTHFPGAQLYWFAESHVQKQESFDCRSPEVPAPF
EVHEHGLRFHAAPGLGHKTGFFADQRDNRRRFAELAKGRRVLDLCCNAGGFAVHAMAAGA
REAVGVDLDAGILEVARANAAANGVEVSFEQSDIFDWVRAAVAGGERYDAVILDPAKLTR
DRSKVMDALKKYFAMNRMALDIIPPGGLLLTCSCTGLVGEGEFLEMLRRVALNAGREIQV
LEVRGAGADHPFRTDVPEGRYLKAVFCRVD