Protein Info for LRK55_RS06370 in Rhodanobacter denitrificans MT42

Annotation: NAD(P)H-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 234 PF02525: Flavodoxin_2" amino acids 5 to 212 (208 residues), 106 bits, see alignment E=2.1e-34 PF03358: FMN_red" amino acids 73 to 121 (49 residues), 28.8 bits, see alignment 8.8e-11

Best Hits

Swiss-Prot: 40% identical to AZOR2_IDILO: FMN-dependent NADH-azoreductase 2 (azoR2) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

KEGG orthology group: K01118, FMN-dependent NADH-azoreductase [EC: 1.7.-.-] (inferred from 70% identity to vpe:Varpa_3168)

Predicted SEED Role

"FMN-dependent NADH-azoreductase"

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (234 amino acids)

>LRK55_RS06370 NAD(P)H-dependent oxidoreductase (Rhodanobacter denitrificans MT42)
MITLLHIDASARGGRSDLHAHGSHTRRLGARFVARWHAVRAADPVFYRDVGQAPPAPVDS
AWIHAAFTPESTRLPWMQGRLAESDRLVDELAAADLIVAGVPMYNFGVPAGFKAYIDNIV
RVGRTFGFDRARRGEPYWPLLTDRPRTLVLLGSRGDYGYGGRIAAMNHVEPAVRTAFGYI
GIRDVRTAAVEYDEFGGECLAASLREAEAAVDALVDELLAGLPGARVKPPALMA