Protein Info for LRK55_RS06185 in Rhodanobacter denitrificans MT42

Annotation: HAD family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 PF00702: Hydrolase" amino acids 5 to 191 (187 residues), 86.4 bits, see alignment E=5.2e-28 PF13419: HAD_2" amino acids 69 to 197 (129 residues), 48.2 bits, see alignment E=2.2e-16 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 100 to 197 (98 residues), 45.6 bits, see alignment E=8.4e-16 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 117 to 191 (75 residues), 43.5 bits, see alignment E=4.2e-15 PF13242: Hydrolase_like" amino acids 152 to 206 (55 residues), 41.5 bits, see alignment E=1.5e-14

Best Hits

KEGG orthology group: K07025, putative hydrolase of the HAD superfamily (inferred from 54% identity to xcv:XCV4079)

Predicted SEED Role

"Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase" (EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (232 amino acids)

>LRK55_RS06185 HAD family hydrolase (Rhodanobacter denitrificans MT42)
MPILALTLDLDDTLWPVLPALERADQAVDAWLRRHHPEVARAWPIAAMRALRMQVAAERL
DLAHDFTRQRQLTLQQAFAACGVDEAPVEALWEIYFAARNRVELYPDSLPALQRITARWP
LASLTNGNADLQRIGIHAHFAHHVCARDSGVAKPDPRIFLAAAERLGVAPGEILHVGDDP
AMDMAGARDAGLRTAWINRAGLPWPAALGQPPELDLRDMTALADWLDAHGAR