Protein Info for LRK55_RS05435 in Rhodanobacter denitrificans MT42

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 PF04321: RmlD_sub_bind" amino acids 1 to 306 (306 residues), 318 bits, see alignment E=7.7e-99 TIGR01214: dTDP-4-dehydrorhamnose reductase" amino acids 2 to 305 (304 residues), 317.7 bits, see alignment E=3e-99 PF01370: Epimerase" amino acids 3 to 173 (171 residues), 60.1 bits, see alignment E=3.5e-20 PF16363: GDP_Man_Dehyd" amino acids 47 to 149 (103 residues), 29.7 bits, see alignment E=7.2e-11

Best Hits

Swiss-Prot: 45% identical to RMLD_ECOLI: dTDP-4-dehydrorhamnose reductase (rfbD) from Escherichia coli (strain K12)

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 57% identity to xal:XALc_2694)

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.133

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (311 amino acids)

>LRK55_RS05435 dTDP-4-dehydrorhamnose reductase (Rhodanobacter denitrificans MT42)
MRILLLGANGQLGRTFLDQGGLAARGELVAASRDGALTHGGPGEIADLSIPASLPALLDR
VRPDVIVNAAAYTAVDRAEQEEALATRVNGEAVGVLGRWAAAHGALVIHYSTDYVFDGRQ
SQPYAVDAPTGPLGAYGRSKLAGEQALRDSGADHLTFRTAWVYAAHGHNFLRTMLRLGAE
RDELRVVADQHGAPTSTALIVAGTLAALDAWQAAEATQRRTLIGTHHLVASGHTSWHGFA
EAIFAGAVGRGLIARAPRVAAITTADYPTPAERPAWSLLDNTGFQRRFGYALPDWQSGLH
DVLDALSRQRA