Protein Info for LRK55_RS05220 in Rhodanobacter denitrificans MT42

Annotation: EAL domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 966 PF13185: GAF_2" amino acids 148 to 293 (146 residues), 36 bits, see alignment E=2e-12 amino acids 348 to 486 (139 residues), 46.6 bits, see alignment E=1.1e-15 PF01590: GAF" amino acids 213 to 292 (80 residues), 25.8 bits, see alignment E=3.5e-09 amino acids 357 to 485 (129 residues), 36 bits, see alignment E=2.4e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 534 to 698 (165 residues), 139.1 bits, see alignment E=5.9e-45 PF00990: GGDEF" amino acids 538 to 694 (157 residues), 167.9 bits, see alignment E=4e-53 PF00563: EAL" amino acids 715 to 949 (235 residues), 230.6 bits, see alignment E=4.6e-72

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (966 amino acids)

>LRK55_RS05220 EAL domain-containing protein (Rhodanobacter denitrificans MT42)
MSASPTIRSSQPPPVARDLAALHAAVERLFEAPDAAGVMDVCELMLDNFGIHGRLRWRRM
DEQPGHLAGQMDLAEDPQGPRTLVLEWADLPLPEALRDRLDWLGRLADIRLHQLAETSHL
YEAISRLALAERLQRALYAIAEQAGADHDMPEMMRSLHAIVSSLMYAENFYIVLYDAATD
TVRFPYYVDIADTDPPPPDQHLPLHGMLHSLTWNLLQEGRPLMGSFEELKQQFGDRFVPI
GPSCEHWLGAPLLRTGRVVGGIVMQSYRADTHYSRHDLELLNYVAQHVQTALERREAHLE
LGRRVTDRTAALREANRVLRQQVLQRQRGERLQAALFRIAELANTSDSLDKFYAAMHQVI
SGLLYARNFYIALIDEENGHLTFPYSVDDMDSVRPPRAHGRGATEYVLRHAKPLLATPAE
IDRLSALGEISLFGARSVCWLGVPLIWGGKAMGVLALQSYSPEHTYNERDQELLTFVSYH
IANALQRKQAATALKQAYAGLERRVTERTRALALANRDLREQIAERERVERRLKYETLHD
SLTGLPNRTLLLQRLEQAMQRYAANPGEQFAVLFIDLDRFKVINDSVGHLVGDDLLFQVG
GRIRACLKTRDVVARLGGDEFAVLLEGIVETGKATLIAERIISELQTPFRLGTKEIFTSA
SIGIALARPHYRQPEELLRDADAAMYNAKDGGRHRAAMFDDRLRREALSLLDMESDLRHA
LSRNEFEPFYQPIVELADGRITGYEALLRWHHPERGLLPPHDFLLIAEECGCAEAIDWQI
FEQVCTQAVRLIGTDGFISINVSGRHFRSADLDLRLLALFDQYAVPTRCIRIEVTEHALL
ENPTQVKQMLQNLRSHGVGIALDDFGTGYSSLSYLHQYPFETLKIDRSFITELPPDDAET
QGLALVRAIQVLADSLRMKVIAEGIEEESQRQALLRVGCRYGQGFLFAEAQPASTWLGAD
KPLLLA