Protein Info for LRK55_RS04710 in Rhodanobacter denitrificans MT42

Annotation: aspartate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 590 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 582 (582 residues), 784.8 bits, see alignment E=2.3e-240 PF01336: tRNA_anti-codon" amino acids 18 to 100 (83 residues), 47.1 bits, see alignment E=2.8e-16 PF00152: tRNA-synt_2" amino acids 115 to 557 (443 residues), 402.2 bits, see alignment E=2.4e-124 PF02938: GAD" amino acids 306 to 404 (99 residues), 89.3 bits, see alignment E=2.4e-29

Best Hits

Swiss-Prot: 72% identical to SYD_STRMK: Aspartate--tRNA ligase (aspS) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 72% identity to sml:Smlt3718)

MetaCyc: 58% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (590 amino acids)

>LRK55_RS04710 aspartate--tRNA ligase (Rhodanobacter denitrificans MT42)
MRTHYCGLIDEALIGQTVTLCGWVNKIRLQAHVAFVDLRDHEGLAQVVIDRDNPAAFAVA
GELGYEYCVRVTGTIRPRVSVNDKLKTGTVELLADTVEVLNAAKDLPFALHENPNEDLRM
TYRYLDLRRPEMQAMMRKRIKLVQALRRYLDARGFQDVETPILTKATPEGARDYLVPSRV
HPGQFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEDLRADRQPEFTQLDLEFAFVEEK
DVQDFVEELIRHVFREVQSVELDAAFPRMTWMEAMRRFGSDKPDLRIALELVDVADAVKH
VEFKVFAEPANDPAGRVAALRVPGGSTLSRKDIDGLAEYVARYGAKGLAWLKVEDLAKGR
DGINSPVAKFLDDAALNGVLKATAAQTGDIVFFGAGAWKTVSDFMGALRLKVGKDRGLVA
DGWKPLWVTDFPMFEYDAEEQRFVALHHPFTAPKVDDAAQLRADPHNAVSRGYDMVLNGN
EIGGGSIRIHRPEMQSTVFELLGIGAEEAEMKFGFLLKALKFGAPPHGGLAFGIDRIAAL
MAGTESIRDVIAFPKTTSAQDLMTDAPSPIAAAQLKELHVRVATDGEAAG