Protein Info for LRK55_RS03655 in Rhodanobacter denitrificans MT42

Annotation: GDP-L-fucose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 PF04321: RmlD_sub_bind" amino acids 9 to 157 (149 residues), 38.4 bits, see alignment E=1.2e-13 PF01370: Epimerase" amino acids 10 to 241 (232 residues), 224.5 bits, see alignment E=2.1e-70 PF16363: GDP_Man_Dehyd" amino acids 42 to 260 (219 residues), 59.6 bits, see alignment E=5.7e-20

Best Hits

Swiss-Prot: 65% identical to FCL1_ARATH: GDP-L-fucose synthase 1 (GER1) from Arabidopsis thaliana

KEGG orthology group: K02377, GDP-L-fucose synthase [EC: 1.1.1.271] (inferred from 68% identity to dvm:DvMF_2695)

MetaCyc: 65% identical to GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase (Arabidopsis thaliana col)
GDP-L-fucose synthase. [EC: 1.1.1.271]

Predicted SEED Role

"GDP-L-fucose synthetase (EC 1.1.1.271)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 1.1.1.271)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.271

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (320 amino acids)

>LRK55_RS03655 GDP-L-fucose synthase (Rhodanobacter denitrificans MT42)
MNLIRTSSRIYVAGHRGMVGSAIVRRLQAEGFNNLALRTRQELDLTDQHAVEAFFATERP
EYVFLAAAKVGGIHANNAYPAEFLRDNLAIQTNVIHSAWKQGAKKLLFLGSSCIYPRDCP
QPIKEEYLLTGPLEPTNEWYAIAKIAGVRTCQAYRRQYGFDAICAMPTNLYGPCDNFDPQ
DSHVLPGLIRRFHEAKLRGDEAVVIWGTGQPLREFMHVDDLADAAMLLMHGYSSEEIVNI
GSGDEVSIAELARLIADAVGFCGRLEFDPSRPDGTPRKRLNISRLNAMDWSARTRLQPGI
AATYAAFLAAQRDPAERITP