Protein Info for LRK55_RS02955 in Rhodanobacter denitrificans MT42

Annotation: phenylalanine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 791 (791 residues), 781.6 bits, see alignment E=4.8e-239 PF01588: tRNA_bind" amino acids 45 to 145 (101 residues), 85.3 bits, see alignment E=6.4e-28 PF03483: B3_4" amino acids 213 to 385 (173 residues), 237.3 bits, see alignment E=2e-74 PF03484: B5" amino acids 407 to 471 (65 residues), 73.8 bits, see alignment E=2.6e-24 PF17759: tRNA_synthFbeta" amino acids 474 to 683 (210 residues), 180.3 bits, see alignment E=9.2e-57 PF03147: FDX-ACB" amino acids 698 to 791 (94 residues), 87.9 bits, see alignment E=1.2e-28

Best Hits

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (792 amino acids)

>LRK55_RS02955 phenylalanine--tRNA ligase subunit beta (Rhodanobacter denitrificans MT42)
MKFSENWLRELVEIQADRAELAHALTMAGLEVEELTPLGDGLAGVVVARIVAAEKHPEAD
RLQVCKVDAGQGEPLQIVCGAPNARVGINVPLATIGATLPGGIRIKAARLRGVESSGMLC
SAKELGIDTDASGLLELPTDAPVGQPLAGYLGLPDASFELKLTPNRPDCLGLVGLAHDVA
ALFGSTVRLPASATVPVTSNARRGIRLEAGKDAPRYLGRTIEGVDPAARSPLWLAERLRR
AGLRPISAVVDVTNYVMLELGQPLHAFDNDTLEGDIIVRHAHAGETLKLLDGNEAKLDES
FVLIADEKKALAVAGVMGGHDSRVSDATRNVFLESAHFAPAAIMGRARKLGLHTDASHRF
ERGVDPELPRRALERASELLLAIAGGQAGPVLAAENPADLPMPAPVALRRVRLQRVLGVD
VAEAEVARIFTALGMQVATTADGWRVIAPSSRFDIEREEDLIEEVARIFGYDNIPTATPA
GALTLAIEPEARIGELALREQLAARGYYEAVNLSFVATDLLADWGFTEHLVPLANPLSAD
LAVMRPSLLPGLVEALRHNRARQQERVRLFEVARVFAEGNPPVETPSLAIVACGAARAEQ
WGEPSRVPDFFDLKGDLDALIAWGGEPRRWSVHADDLPGWLHPGRGARVARDGLTVGYLG
ALHPQLAKALDLGPDVHVLELALEPLLTRRLPRAQAVPRFPAVRRDIAVDLPEAVRWSQI
EQVVRGTLGERLRELRLFDRYRGKGVEAGRKSLAMGLILQDASRTLTDDDADRCVRDAIA
ALEQSCKAKLRG