Protein Info for LRK55_RS02535 in Rhodanobacter denitrificans MT42

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 879 TIGR00396: leucine--tRNA ligase" amino acids 17 to 878 (862 residues), 1131.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 27 to 237 (211 residues), 100 bits, see alignment E=2.7e-32 amino acids 433 to 589 (157 residues), 35.1 bits, see alignment E=1.4e-12 amino acids 636 to 676 (41 residues), 27.6 bits, see alignment 2.6e-10 PF09334: tRNA-synt_1g" amino acids 51 to 183 (133 residues), 63.5 bits, see alignment E=3.2e-21 PF13603: tRNA-synt_1_2" amino acids 233 to 419 (187 residues), 227.2 bits, see alignment E=2.3e-71 PF08264: Anticodon_1" amino acids 717 to 842 (126 residues), 67.7 bits, see alignment E=2.1e-22

Best Hits

Swiss-Prot: 63% identical to SYL_PSEAB: Leucine--tRNA ligase (leuS) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 75% identity to psu:Psesu_2237)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (879 amino acids)

>LRK55_RS02535 leucine--tRNA ligase (Rhodanobacter denitrificans MT42)
MQDIQAPTSPEQDEAAYRPQAVEAAAQRYWNAQRAYEVKEDASRPKFYCLSMLPYPSGAL
HMGHVRNYTIGDVISRYQRMNGKNVLQPMGWDAFGLPAENAAIKNATAPAKWTYANIEHM
RSQLKSLGYAIDWSREFATCRPEYYRWEQLMFTRLLKQGLAYRKNAVVNWDPVDQTVLAN
EQVVDGRGWRSGAVVEKREIPQWFLKITDYAPELLDGLDTLPGWPDAVKTMQRNWLGRSE
GLEIHFAVDGEAAPLTVFTTRPDTLMGVTFVSIAGEHPLAHKAARGNPPLAAFLDELKHG
GVSEAELETQDKRGMATGLYAIHPVSGERVPVWVANFVLMGYGTGAVMAVPGHDERDFAF
AHKYGLPIKQVIAAGDESYDPAVWQDWYSDKTRVDMRVMNSGAMDGKDYRAAFDHIAGVL
EADGKGQRRVNWRLRDWGVSRQRYWGCPIPVIYCPKCEAVPVPEDQLPVVLPEDVAFSGV
QSPIKADPQWRKTTCPQCGGAAERETDTFDTFMESSWYYARYTSPGANAQIDDRANYWLP
VDQYIGGIEHAIMHLLYFRFYHKLLRDAGMVHSDEPAQNLLCQGMVIAETFYRDNADGSK
EWINPAAVEIRRDDKARVIGATLKADGQPVHIGGTEKMSKSKNNGIDPQTMVDKYGADTV
RLFSMFAAPPEQSLEWSEAGVEGMARFLKRLWREVTTHAAGPDHAVTDPATLDAGQKALR
RQLHETIQKVSDDFGRRHAFNTAIAALMELLNALGKFNDQSEQGRAVRHEALEAMVLLLN
PVVPHVSHALWQVLGHAESVLEDQPWPPVDPAALVRDTVTLAVQINGKLRATIELAAQAS
KEEAEALALAQPQVAHFLAGMTVRKVIVVPGKIVNIVAG