Protein Info for LRK55_RS02180 in Rhodanobacter denitrificans MT42

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 TIGR00435: cysteine--tRNA ligase" amino acids 4 to 452 (449 residues), 484.1 bits, see alignment E=2.6e-149 PF01406: tRNA-synt_1e" amino acids 16 to 314 (299 residues), 392.9 bits, see alignment E=2.2e-121 PF00133: tRNA-synt_1" amino acids 221 to 305 (85 residues), 29.6 bits, see alignment E=6.3e-11 PF09190: DALR_2" amino acids 343 to 397 (55 residues), 38.1 bits, see alignment 3.6e-13

Best Hits

Swiss-Prot: 66% identical to SYC_XANC5: Cysteine--tRNA ligase (cysS) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 66% identity to xcv:XCV2553)

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>LRK55_RS02180 cysteine--tRNA ligase (Rhodanobacter denitrificans MT42)
MPISLYNSLTRRTEPFAPLDPERVTIYLCGPTVYNYVHIGNARGPVVFDVLVRLLRRHYP
QVAYARNITDVDDKINAAAQQQGVPIGTITERFAQAYREDMAVLGVAPPDIEPHATTHIG
PIIAMIESLLDGGHAYAAEGHVLFDVGSFPAYGELSGRDTDELIAGSRVDVAPYKRSPGD
FVLWKPSTPELPGWDSPWGRGRPGWHIECSAMSAAHLGETIDIHAGGIDLLFPHHENEIA
QSTCAHGGKTFARWWMHNGMLTFDGRKMSKSLGNVLLLHDLLELHSPEALRLRLLSGHYR
QPLDWSEAAIAQATSTLDGWYRVLRDLAMVELPPGELPVPERIETALCDDLNTPQALAEL
SVLADAARQSGSAAAKAALLGGGALLGLLQQDAEAWFRRGESTVDAAHIEDLLQQRQAAR
AARDFARADAIRDELAAMHIAIEDSAQGTRWSIAKP