Protein Info for LRK55_RS01515 in Rhodanobacter denitrificans MT42

Annotation: 50S ribosomal protein L3 N(5)-glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 16 to 298 (283 residues), 385.4 bits, see alignment E=1.7e-119 TIGR00536: methyltransferase, HemK family" amino acids 18 to 303 (286 residues), 235.7 bits, see alignment E=5.6e-74 PF05175: MTS" amino acids 132 to 218 (87 residues), 64.4 bits, see alignment E=2.1e-21 PF06325: PrmA" amino acids 137 to 211 (75 residues), 27.2 bits, see alignment E=5.3e-10 PF13847: Methyltransf_31" amino acids 139 to 268 (130 residues), 30.4 bits, see alignment E=6.3e-11 PF13649: Methyltransf_25" amino acids 141 to 220 (80 residues), 29.7 bits, see alignment E=1.8e-10

Best Hits

Swiss-Prot: 66% identical to PRMB_XANCP: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 66% identity to psu:Psesu_1950)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (319 amino acids)

>LRK55_RS01515 50S ribosomal protein L3 N(5)-glutamine methyltransferase (Rhodanobacter denitrificans MT42)
MTGQSAAPWIAVTAELATIIDFIRYGASRFSAAGLTFGHSHDNPIDEATHLVLASLHLPP
DIPPAYGAGRLTAAERANVLALIERRVSERLPVAYLVGETWFAGLKFKSDRRALVPRSPI
AELIESGFAPWLDERHIERALDLCTGSGCIGIAMAEYNPEWLVDIVDISDEALSLARENI
AFQHVEGRVEAIRSDLFAGVAGRKYDLIVSNPPYVTEDEYAALPGEYAHEPKLGLTSGVD
GLDLCLRMLDEAADHLTDDGLLIVEVGESEHALAALLPEVPFVWIEFKVGPMGVFALERR
DLVEHAAAIRAAAAARRPG