Protein Info for LRK55_RS01375 in Rhodanobacter denitrificans MT42

Annotation: bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 TIGR01656: histidinol-phosphate phosphatase domain" amino acids 4 to 143 (140 residues), 103 bits, see alignment E=2.2e-33 TIGR01261: histidinol-phosphatase" amino acids 4 to 161 (158 residues), 195.8 bits, see alignment E=5.1e-62 TIGR01662: HAD hydrolase, family IIIA" amino acids 4 to 146 (143 residues), 68 bits, see alignment E=1.4e-22 PF13242: Hydrolase_like" amino acids 103 to 160 (58 residues), 33.2 bits, see alignment E=4e-12 PF00475: IGPD" amino acids 195 to 336 (142 residues), 204.8 bits, see alignment E=6.4e-65

Best Hits

Swiss-Prot: 64% identical to HIS7_STRM5: Histidine biosynthesis bifunctional protein HisB (hisB) from Stenotrophomonas maltophilia (strain R551-3)

KEGG orthology group: K01089, imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase [EC: 3.1.3.15 4.2.1.19] (inferred from 66% identity to psu:Psesu_1817)

MetaCyc: 54% identical to imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase (Escherichia coli K-12 substr. MG1655)
Imidazoleglycerol-phosphate dehydratase. [EC: 4.2.1.19]; Histidinol-phosphatase. [EC: 4.2.1.19, 3.1.3.15]

Predicted SEED Role

"Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)" in subsystem Histidine Biosynthesis (EC 3.1.3.15, EC 4.2.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.15

Use Curated BLAST to search for 3.1.3.15 or 4.2.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (354 amino acids)

>LRK55_RS01375 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB (Rhodanobacter denitrificans MT42)
MSRKILFVDRDGCLIEEPADEQIDSYEKLALLPGVIAALQRFVAAGYELVMVSNQDGLGS
DSFPEPHFSGPHELLLRILASQGIAFREVLIDRSLPHEGLDTRKPGVGMLRHYLADDGWS
RAASAMVGDRETDLQFAANMGVRGLRVGPQGVGWEELAHQLLDAPRVATVVRNTRETRIT
VSVDLDQVAEPQAHTGLGFFDHMLEQIGKHGGFALQLDCDGDTHIDEHHTIEDCALALGQ
ALKQALGDKRGIGRYGFTLPMDEAQASAALDLSGRPYFVFEGSFPRERVGEVPTELVPHF
FRSLCETLGANLHLAVRGDNAHHMVEACFKVVARTLRQAIARSGSELPSTKGSL