Protein Info for LRK55_RS00780 in Rhodanobacter denitrificans MT42

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 857 TIGR01063: DNA gyrase, A subunit" amino acids 9 to 837 (829 residues), 1221.3 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 506 (475 residues), 561.9 bits, see alignment E=1e-172 PF03989: DNA_gyraseA_C" amino acids 537 to 584 (48 residues), 44 bits, see alignment 1.2e-15 amino acids 587 to 636 (50 residues), 44.9 bits, see alignment 6.1e-16 amino acids 644 to 687 (44 residues), 32.5 bits, see alignment 4.5e-12 amino acids 692 to 737 (46 residues), 39.2 bits, see alignment 3.8e-14 amino acids 741 to 787 (47 residues), 38.4 bits, see alignment 6.5e-14 amino acids 792 to 837 (46 residues), 46.6 bits, see alignment 1.8e-16

Best Hits

Swiss-Prot: 65% identical to GYRA_PECCA: DNA gyrase subunit A (gyrA) from Pectobacterium carotovorum

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 65% identity to dze:Dd1591_3019)

MetaCyc: 64% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (857 amino acids)

>LRK55_RS00780 DNA gyrase subunit A (Rhodanobacter denitrificans MT42)
MAELAKEVIRVNIEDEMRQSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNELGNVWN
KPYKKSARVVGDVIGKYHPHGDQSVYDAIVRMAQPFSLRYLLVDGQGNFGSVDGDSAAAM
RYTEVRMSRLTHELLADIDKETVDFGPNYDESEHEPLVLPTRVPNLLVNGSAGIAVGMAT
NIPPHNLNEVIAATIALIDEPTLSIDELMHYIPGPDFPTHGIINGSSGIIEAYRTGRGRI
LVRAKAEIETESNGRETIIVHELPYQVNKARLIEKIAELVKEKKLEGISELRDESDKDGM
RVVIEIRKDAMGDVVLNNLFQQTQLQVTFGINMVALLDGQPKLLNLKDILEAFIRHRREV
VTRRTIFELRKARARAHILEGLTVALANIDEMIELIKTSSSPAEARERMVARRWEAGLVR
ALLSATGADASRPEDMDPRDGLKDDGYQLSEAQALEILAMRLHRLTGLEQEKLSDEYRQI
LETIRGLIEILENPNRLLEVIREELEAVKAEFGDARRTEIQHSQEDLNVLDLIAPEDMVV
TLSHTGYVKRQPASTYRAQRRGGKGRSASALKDEDVVEQLWVVNTHDTLLTFTSTGRVYW
LKVYQIPEAGPGARGKPIINLLPLGEGEKVQAVLPVREYSDDRYVFFATRHGTVKKTPLT
EFAYQLQKGKQAIKLDEGDALVNVELTDGNSDILLFASNGKVNRFDENTVRSMGRTATGV
RGMKLVGGAEVVSLIVAAEGDILTATARGYGKRTELAEFAKKGRGTQGVIGIQCSERNGA
LVAAVQVTEAHELMLISNQGTLVRTRVAEVSQLGRNTQGVTLIRLPADETLVSVMRLDAE
EDNGEDAEVVEPSVANE