Protein Info for LRK55_RS00560 in Rhodanobacter denitrificans MT42

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 17 to 907 (891 residues), 1370 bits, E=0
• PF00330: Aconitase
  • amino acids 66 to 580 (515 residues), 540.6 bits, E=3.8e-166
• PF00694: Aconitase_C
  • amino acids 710 to 837 (128 residues), 154.8 bits, E=1.6e-49

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• nitrogen remobilization from senescing leaves (7/8 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• mixed acid fermentation (12/16 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• reductive TCA cycle I (6/11 steps found)
• methylaspartate cycle (11/19 steps found)
• reductive TCA cycle II (5/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (913 amino acids)

>LRK55_RS00560 aconitate hydratase AcnA (Rhodanobacter denitrificans MT42)
MKDTFSVRDTLEVNGKKYAFASLTKLGQRFDLKRLPYSMKILLENLLRHEDGVDVTAKEI
EAVATWDSKKEPDTEIAFMPARVLLQDFTGVPCVVDLAAMRDAMKMLGGDPTLINPLSPA
ELVIDHSVQVDVFGSEDALERNVEMEFKRNQARYSFLRWGQKALTDFKVVPPRTGIVHQV
NLEYLARVVMGTEIEGQLWAYPDTLFGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPSS
MLIPQVVGLKLLGQLPEGATATDLVLTVTELLRRQGVVGKFVEFFGPGLKHLALADRATL
GNMAPEYGATCAICPIDVESLKYLRLSGRSEEQVALVEAYAKVQGLWHDENSAPAEFSVT
LELDLASVKPSMAGPKRPQDHVLLTNVKQNFNDNLGATIVRRNGAEARFANEGGATAVGH
DASARTHRQRVVRNGQEFHVDDGAVVIAAITSCTNTSNPAVMLAAGLLAKKAAAKGLKTK
PWVKTSLAPGSKVVTDYLEKTGLLTELDKLGFYLVGYGCTTCIGNSGPLPEEVSKAISDG
DLTVGAVLSGNRNFEGRVHAEVKMNYLASPPLVVAYALAGSLDVDLTTEPLGQGTDGKNV
YLKDVWPSNKEISDLMAGAVTSDMFKKNYADVFKGDERWSSIASPDGDLYAWDRDSTYIK
NPPYFDGMTMALTPVEDIHAARCLGLFGDSITTDHISPAGAIKKDSPAGRFLISRGVQPI
DFNSYGSRRGNDDVMVRGTFANIRIKNQLLDGVEGGFTRHIPSGEQMSIYDAAMKYKEEK
TPLVVIAGKEYGTGSSRDWAAKGTLLLGIKAVIAESFERIHRSNLVGMGVLPCTFKDGEN
AKSLGLTGNETFDLTGLDNGNSRNATVTATAADGSRKQFSVNVMLLTPKEREFFRHGGIL
QYVLRQLAGKKAA