Protein Info for LRK55_RS00325 in Rhodanobacter denitrificans MT42

Annotation: type I restriction endonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1026 PF04313: HSDR_N" amino acids 118 to 215 (98 residues), 47.2 bits, see alignment E=5.1e-16 PF04851: ResIII" amino acids 287 to 442 (156 residues), 45 bits, see alignment E=2.3e-15 PF18766: SWI2_SNF2" amino acids 290 to 539 (250 residues), 87.1 bits, see alignment E=2.9e-28 PF00270: DEAD" amino acids 307 to 454 (148 residues), 28.2 bits, see alignment E=3.1e-10

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 81% identity to aeh:Mlg_1789)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1026 amino acids)

>LRK55_RS00325 type I restriction endonuclease (Rhodanobacter denitrificans MT42)
MPAHTETAFEAAIELGLLASGGYGKRNPTDYDEALALFPDDVIGFIQDSQAKRWSQLEAL
LANKTRATVLDCLAKELVSKGTLQVLRHGFKCYGKSLRLAYFRPNSAMNPDSAALYAMNR
LTITRQVAFTSALKRPDGAHRRCILDVTLAVNGLPVVTAELKNPLTGQRAADAVTQYQRD
RDERDLIFAFKQRALVHFAVDPDEAWMTTRLKGRETVFLPFNRGFAHGAGNPPVAHDWKT
HYLWDEVLKADSLLDILQRFMHLEVVERKVKTDKGARTIRRETMIFPRYHQLDAVRRLVG
HARQHGSGHNYLVQHSAGSGKSNSIAWLAHRLASLHDANDEKVFHSVVVITDRRMLDAQL
QATIYQFEHKTGVVEKIDENTQQLVRALSQGTPIVITTIQKFPFITQALSTLEKKGDGVA
IDTVGRRFAVIVDEAHSSQSGDTASVLRGLLNKDGIEAAIAAQLSDEEDDDLSEEAKKTL
LRETMRRGRQPNLSFFAFTATPKFKTLALFDELGPSGAAPFHEYSMRQAIEESFIMDVLD
NYTTYKRFYGLIKQVEGDPEVPRRKAAKALAQYMELHAVNIDQVVSVIVEHFRLHVMQEI
GGRAKAMVVTGSRLAAVKYKLAFDKYIRDHGYDGIRSLVAFSGTVEDPEDPGSSYTEVAM
NDGLSESELPETFEGDDYRVLLVAEKYQTGFDQPLLQTMYVVKRLAGVQAVQTLSRLNRR
ATGKARTFVLDFVNEEDDIHKAFKPYYESTPVGENADPQRLNELQYELLKWAIFAPSDIT
SFAAVWYKGKREQSANDHREMNAVLDAVVQRFGERDEEDQEAFRGQLTAFRNLYAFLSQI
IPYQDSELEKFYTFVRNLISKLPPPGGGRAYALDDEVALRYYRLQQLTDGSIDLHEGEAY
PLKGPTDLGTGAVKDEEVPLSTLVEKLNERFGTDFTRADQLFFDQIRVSAEDNEKIVEAA
KANNLTNFAAFLEKALDELFIDRMEGNEDIFSRVMTDKAFRNAAQEHLAREIFERVRKGD
ERNSES