Protein Info for LRK54_RS18115 in Rhodanobacter denitrificans FW104-10B01

Annotation: bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 358 PF02805: Ada_Zn_binding" amino acids 18 to 81 (64 residues), 106.7 bits, see alignment E=9.8e-35 PF00165: HTH_AraC" amino acids 108 to 138 (31 residues), 31.4 bits, see alignment (E = 3.3e-11) PF12833: HTH_18" amino acids 112 to 189 (78 residues), 64.9 bits, see alignment E=1.4e-21 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 276 to 355 (80 residues), 113.6 bits, see alignment E=1.6e-37 PF01035: DNA_binding_1" amino acids 277 to 356 (80 residues), 116.4 bits, see alignment E=8.6e-38

Best Hits

Swiss-Prot: 54% identical to ADA_ECOLI: Bifunctional transcriptional activator/DNA repair enzyme Ada (ada) from Escherichia coli (strain K12)

KEGG orthology group: K10778, AraC family transcriptional regulator, regulatory protein of adaptative response / methylated-DNA-[protein]-cysteine methyltransferase [EC: 2.1.1.63] (inferred from 73% identity to mpt:Mpe_A3753)

MetaCyc: 54% identical to DNA-binding transcriptional dual regulator / DNA repair protein Ada (Escherichia coli K-12 substr. MG1655)
2.1.1.M37 [EC: 2.1.1.M37]; Methylated-DNA--[protein]-cysteine S-methyltransferase. [EC: 2.1.1.M37, 2.1.1.63]; 2.1.1.63 [EC: 2.1.1.M37, 2.1.1.63]

Predicted SEED Role

"ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63

Use Curated BLAST to search for 2.1.1.63 or 2.1.1.M37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (358 amino acids)

>LRK54_RS18115 bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada (Rhodanobacter denitrificans FW104-10B01)
MKTPEKQAARAATLADPRWAAVQARDAHADGSFFYSVRSTGVYCRPSCAARAARPENVAF
HATAAEAERAGFRPCRRCRPDQPSLAQQQAAMVTAACRLIEQAETVPTLEQLAQPSGLSP
FHFHRVFKAVAGVTPKQYATAHRNRRVRSGLERSHTVTEAIFDAGYNASSRFYETANEVL
GMTPSRYRAGGADSEIRFAIGACSLGAILVAQSARGVCAILLGDDPDALARELQDRFPRA
RLIGGDRDYEHLVAQVVGFVEAPALGLDLPLDVRGTAFQQRVWQALREIPVGSTASYSEI
ARRIGAPKAARAVAQACAGNLLAVAIPCHRVVRNDGDLSGYRWGVPRKRALLEREAQA