Protein Info for LRK54_RS18000 in Rhodanobacter denitrificans FW104-10B01

Annotation: carbamoyl-phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1054 (1053 residues), 1542.1 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 238.2 bits, E=3.3e-74
  • amino acids 674 to 875 (202 residues), 139.2 bits, E=6.6e-44
• PF02787: CPSase_L_D3
  • amino acids 428 to 506 (79 residues), 83.6 bits, 3.5e-27
• PF02655: ATP-grasp_3
  • amino acids 674 to 847 (174 residues), 23.2 bits, E=2.9e-08
• PF02222: ATP-grasp
  • amino acids 683 to 847 (165 residues), 45.5 bits, E=2.9e-15
• PF15632: ATPgrasp_Ter
  • amino acids 807 to 874 (68 residues), 29.5 bits, 2.5e-10
• PF02142: MGS
  • amino acids 958 to 1043 (86 residues), 73.9 bits, E=4.2e-24

Best Hits

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 70% identity to aaa:Acav_2592)

MetaCyc: 70% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-citrulline degradation (2/3 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine degradation V (arginine deiminase pathway) (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (3/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1075 amino acids)

>LRK54_RS18000 carbamoyl-phosphate synthase large subunit (Rhodanobacter denitrificans FW104-10B01)
MPKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALKEEGFRVILVNSNPATIMTDPET
ADAVYIEPINWQTVERIIAKERPDAVLPTMGGQTALNCALDLADHGVLEKYQVELIGARR
EAIRMAEDRELFKQAMTEIGLESPKSDVAKSFEHAVEIQARLGFPVIIRPSFTLGGTGGG
IAYNKEEFELIVKRGLEMSPTHEVLIDESVLGWKEFEMEVVRDTADNCIIICAIENLDPM
GVHTGDSITVAPAQTLTDKEYQRLRDASIAVLRKIGVDTGGSNVQFGVNPVDGRVVVIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNDITGGLTPASFEPSIDYVVTKIP
RFAFEKFPAADARLTTQMKSVGEVMAIGRSFHESLQKALRGLEIGKTGLNPTGLDLGTDD
GLAVLKRELREPRPDRVFHLADAFRAGLSLEEVFNLSRVDPWFLAAFEDIVLTEAEITRQ
GITALDAPRLRELKRLGFADARIAELIGTDEAAVRHLRHTLGVRPVYKRVDSCAAEFATS
TAYMYSTYEEECEAAPTGRDKIIVLGGGPNRIGQGIEFDYCCVHASLALREDGYETIMVN
CNPETVSTDYDTSDRLYFEPLTLEDVLEIVHVEKPKGVIVQYGGQTPLKLARALEAAGVP
IIGTSPDSIDLAEDRERFQHMIEKIGLKQPPNRTARNADEALALAREIGYPLVVRPSYVL
GGRAMEVVHDDADLSRYIRDAVKVSNDSPVLLDRFLDHAVEVDVDVIADAEGTVLIGGIM
EHIEEAGVHSGDSSCSLPPYSLSTSIQDEMRRQVSAMAKELKVIGLMNTQFAIQGDTVYI
LEVNPRASRTVPFVSKATGVPLAKIAARVMAGRSLISLGATREVIPAYYSVKEAIFPFLK
FQNVDPILGPEMRSTGEVMGVGRSFGAAFARGHEAAGIKAPTVGKAFLSVRDGDKDRLLP
VAREIIARGFNLVATAGTASYLTGHGVACERINKVLEGRPHIVDLIKNGEIVYIVNTTEG
KQAIADSFSIRREALQHRVTYSTTVAGARALVHSLDFHGDGEVHSLQELHKELSA