Protein Info for LRK54_RS17330 in Rhodanobacter denitrificans FW104-10B01
Annotation: serine--tRNA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 72% identical to SYS_XANCP: Serine--tRNA ligase (serS) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
KEGG orthology group: K01875, seryl-tRNA synthetase [EC: 6.1.1.11] (inferred from 72% identity to xca:xccb100_2666)MetaCyc: 65% identical to serine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Serine--tRNA ligase. [EC: 6.1.1.11]; 6.1.1.11 [EC: 6.1.1.11]
Predicted SEED Role
"Seryl-tRNA synthetase (EC 6.1.1.11)" in subsystem Glycine and Serine Utilization (EC 6.1.1.11)
MetaCyc Pathways
- tRNA charging (21/21 steps found)
- L-selenocysteine biosynthesis I (bacteria) (1/3 steps found)
- L-selenocysteine biosynthesis II (archaea and eukaryotes) (1/4 steps found)
- superpathway of chorismate metabolism (40/59 steps found)
- shinorine biosynthesis (3/8 steps found)
- vanchrobactin biosynthesis (2/8 steps found)
- enterobactin biosynthesis (2/11 steps found)
- colibactin biosynthesis (7/38 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.1.1.11
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (426 amino acids)
>LRK54_RS17330 serine--tRNA ligase (Rhodanobacter denitrificans FW104-10B01) MLDPALLRSRLAETAARLAETRGYTLDVAAVESLENARKQLATETQDLQNLRNTRSKSIG QAKARGEDTASLMAEVAGIGDKLKANEQALTEVQAKLADIALGIPNIPHASVPVGKDEKD NVEVSRWGTPREFAFTVKDHVELGERHGWLDGDTGAKLSGARFTVLRGQLAHLHRALGQF MLDLHTAEHGYLECNVPVLVNADSMQGTGQLPKFEEDLFATEVGETKRYLIPTAEVSLTN LARNTIQDADALPLRMTAHTLCFRAEAGSYGRDTRGMIRQHQFEKVEMVQITHADQSHAQ LEEMVGHAEKVLQKLGLPYRKVLLCSGDMGFTAVKTYDLEVWLPSQQTYREISSCSNCED FQARRLQARVRNPDTGKPELVHTLNGSGLAIGRTLIAVMENYQNADGSIDVPEVLRPYMA GLDSIA