Protein Info for LRK54_RS17315 in Rhodanobacter denitrificans FW104-10B01

Annotation: ligand-binding sensor domain-containing diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1012 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details transmembrane" amino acids 802 to 823 (22 residues), see Phobius details PF07494: Reg_prop" amino acids 52 to 74 (23 residues), 20.7 bits, see alignment (E = 4.8e-08) amino acids 234 to 252 (19 residues), 16.9 bits, see alignment (E = 8.3e-07) amino acids 326 to 348 (23 residues), 17.8 bits, see alignment (E = 4.3e-07) amino acids 374 to 393 (20 residues), 17.2 bits, see alignment (E = 6.9e-07) PF07495: Y_Y_Y" amino acids 726 to 791 (66 residues), 40 bits, see alignment 4.6e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 849 to 1011 (163 residues), 155.2 bits, see alignment E=6.2e-50 PF00990: GGDEF" amino acids 853 to 1007 (155 residues), 146.7 bits, see alignment E=7.8e-47

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1012 amino acids)

>LRK54_RS17315 ligand-binding sensor domain-containing diguanylate cyclase (Rhodanobacter denitrificans FW104-10B01)
MSDAHRRRRRFRLPLAGLFLLFLLGSAARADSGDPWAPFDAPWFDQVGISDGLPHSVTTA
IAQDRDGLIWIGTMGGLVRYDGYRLQVFGAGSGKAPGLPDAYVRSLLALPDGSLLIGTNT
GGVARFEQADNSFRVYPVGAGGLSDRKIYALGEDHAGGVWIATENGLNHLDLRTDAIRRV
DTGPDTSPRNFSVLQDRHGNLWLGNNNGLFVRRAGSDVFVRPSTPDGPAAAVLGNQIWSV
MQDREGRLWAGSVQAGAAYRDTDGRWHGVPGFSGYPDGARHSTVRDLLQTAGGAVWIATD
GGGVLEYTPGDTGVRRIDHDPAVPSSLPGDSVRALLRDRSGNIWAATDLGVAHHNPQARI
AFSLLPSPLEGNALSDTNVHSIFVDSRGRIWLGLGAGHIDVIDLKAGRMRHLHLDGSQTQ
RDVRSLAEVDGSIWVGTQGLARIHPDTLAIADSVLPALHDKPVLSLRRDGTRLLIGTYDG
LYRYDTRSGTLEHASHVPSDPASLASDTVRYIAQVGDDWWYGTSRGLSIARGDSLPRRFI
NLRHRADDSASLPQDLIGSISRGPHGQVWVSTFDGLGIVDPPVDGESWRFRTVGPAQGLS
SNKVTAVLADDHGRFWASLSNGVARIDGDTHEAISLGTRDGLHIASYVNVAAARAPGGEL
LFGGLGGLTVIRPHWQPSPVATTPLAITNAVVNGVAMPFGKLPASGAKVTLDRGNRNLRL
DFALLDYQAPMETAYSYRMDGLDEDWTNIPKGSLPSAIYTSLPHGQYRLRLRAATRGLQP
RTVETDLALTVKPRWYETTLSRVVAALLLAALIATLVHLRTLYLGRQAAQLQRQVDERTR
DLLVANRRLDELASTDGLTGVYNRRRFLELARLERERAQAGPSCIALFDLDRFKLINDTH
GHLAGDAVIRGAIEVIRQHCRQGDLVGRYGGEEFVLCLPDTPLSLAREIAERICAALAAT
TVNHDGRPIPVTASIGIAALRPGESIEQWLSRADKALYEAKRAGRNRCAVAS