Protein Info for LRK54_RS16935 in Rhodanobacter denitrificans FW104-10B01

Annotation: bifunctional diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 855 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 418 to 578 (161 residues), 155.7 bits, see alignment E=4.6e-50 PF00990: GGDEF" amino acids 419 to 574 (156 residues), 167.8 bits, see alignment E=1.8e-53 PF00563: EAL" amino acids 596 to 830 (235 residues), 253.1 bits, see alignment E=2.4e-79

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (855 amino acids)

>LRK54_RS16935 bifunctional diguanylate cyclase/phosphodiesterase (Rhodanobacter denitrificans FW104-10B01)
MSSGILVVAAQRELRRSLFDALDQAGYPQIHSARDVSHAAILLEGRASLPPLQLIVAVLS
GDEQQARISCEQLHRLPGGTDTPLIVVLGEDATLSAADLPADIADWLSAAQIGSELVPRW
RRWQAGNGQRPRPLAATSDGSAHEDYRYVFEEGDSEWLIADAQTGRLLEVSPTVARHSRM
HASQWEGLPLAEVLRFEGITIDQVLAEANRGWYPCQRKSAQGADTGQASVRRIRHAGREA
LALMFRSDRADLRAEAALSLLSRIFASTSGVDAQTASGRLLFDELGLDYLAVWSARQDGA
DAPAQMLQLWNGDAPAWPPAQLQSSLQLVLGGKPILYRTDAKRLASVDPLLQQLDLAGFA
GLPLYDERHTVLGAMLAGSRRGFGKMGIIEPVLRCAAARFAQMLELGRTREQGRAEGLVD
ALTGLPNRLLFNDRLDTIIREATRNGECFAVLFVDLDRFKAINDTYGHAAGDQVLRSVTQ
RLCGSIRASDTVARYAGDEFTVVLRHIVKNDDVLRVAEKIVQVMETPLFLEDGTELQVTA
SMGLSFFPDDAGDAQTLLKHADEAMYAAKHQGRNTFQIYEVSPEYAQQHGIALKTRLRHA
EGNGELRVVYQPQVNTETEDIMGMEALVRWEHPELGMISPAVFIPLAEESGLIVSIGEWV
MRTACRQAKEWEQRYGLRLRLGVNLSAVQLMEPQLLDTVAAVLRETGLDPTLLEMEVTES
ISIKEAPNLVENLHALHRLGCHIAIDDFGTGAASLDYLRRLPADRIKIDQSFVRNIGVDP
DDEAIVRATVEMAHRLKRAVVAEGVEIEQHLQFLRQHGCDELQGYLFCRPLPPVSFDKLL
AERQRLLEARVAEPA