Protein Info for LRK54_RS16655 in Rhodanobacter denitrificans FW104-10B01

Annotation: protein-L-isoaspartate(D-aspartate) O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 22 to 224 (203 residues), 200.2 bits, see alignment E=1.8e-63 PF01135: PCMT" amino acids 23 to 223 (201 residues), 187.1 bits, see alignment E=7.2e-59 PF13649: Methyltransf_25" amino acids 96 to 165 (70 residues), 28.3 bits, see alignment E=4.8e-10 PF08241: Methyltransf_11" amino acids 97 to 167 (71 residues), 21.8 bits, see alignment E=4.7e-08

Best Hits

Swiss-Prot: 59% identical to PIMT_XANCB: Protein-L-isoaspartate O-methyltransferase (pcm) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 62% identity to xal:XALc_1158)

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (226 amino acids)

>LRK54_RS16655 protein-L-isoaspartate(D-aspartate) O-methyltransferase (Rhodanobacter denitrificans FW104-10B01)
MTTYPLPAADLKGEGMTSQRARDRLAATLKDGGIRDLRVIEVIRNLPRHHFIDQALHSRA
YENDALPIGHGQTISQPWVVARMTEALLEFGVPQKVLEIGTGSGYQAAVLAALVPQVFTV
ERIEALLRQARRRFRQLGLANLRSRYDDGKLGWPDEAPFDAIILTAAGDTIPTRILDQLS
PTGVLVAPVGSPSRQTLIRMHGDGKGDFIQEELGAVSFVPLLGGIG