Protein Info for LRK54_RS16595 in Rhodanobacter denitrificans FW104-10B01

Annotation: cysteine synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 40% identical to DCSD_STRLA: O-ureido-L-serine synthase (dcsD) from Streptomyces lavendulae

KEGG orthology group: None (inferred from 67% identity to rxy:Rxyl_0763)

MetaCyc: 40% identical to O-ureido-L-serine synthase (Streptomyces lavendulae lavendulae)
RXN-14458 [EC: 2.6.99.3]; Cysteine synthase. [EC: 2.6.99.3, 2.5.1.47]

Predicted SEED Role

"Cysteine synthase (EC 2.5.1.47)" in subsystem Cysteine Biosynthesis or Methionine Biosynthesis (EC 2.5.1.47)

MetaCyc Pathways

superpathway of sulfate assimilation and cysteine biosynthesis (8/9 steps found)
L-cysteine biosynthesis I (2/2 steps found)
seleno-amino acid biosynthesis (plants) (3/5 steps found)
D-cycloserine biosynthesis (2/6 steps found)
superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.47

Use Curated BLAST to search for 2.5.1.47 or 2.6.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (316 amino acids)

>LRK54_RS16595 cysteine synthase family protein (Rhodanobacter denitrificans FW104-10B01)
MATRFTTVSDSILDAVGDTPLLEIEGVYAKIEFLNPSGSIKARIAKYMIERAEQEGLLKP
GDTIVEATSGNTGNALSMVAAVKGYRMLVVMPEGLSSERVAISRAYGADVLFCGNFHVNE
ALVRAQRLGQQAGYFCPSQFESEWNVEENRLILGPEILAQLPPGRVPDALVLGVGTGGTL
IGVGQAFREVNPNVRLFAMEPSESCTILCGEIAAHTIEGISDGFVPGIFQRHAGLVNEVL
SVSSTDAVSEMKRLARSYGLFCGPSSGAHLLAAKRIRQNFPELKTVVTVFCDEGEKYLHE
YFMQPAAVDVGATHFR