Protein Info for LRK54_RS15310 in Rhodanobacter denitrificans FW104-10B01

Annotation: methylmalonyl-CoA mutase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02310: B12-binding
  • amino acids 21 to 124 (104 residues), 63.5 bits, E=3.5e-21
• PF03308: MeaB
  • amino acids 206 to 373 (168 residues), 132.3 bits, E=3.5e-42
• PF02492: cobW
  • amino acids 208 to 375 (168 residues), 25.6 bits, E=1.8e-09
• PF01642: MM_CoA_mutase
  • amino acids 661 to 791 (131 residues), 84.9 bits, E=9.7e-28
  • amino acids 852 to 1219 (368 residues), 310 bits, E=5.9e-96

Predicted SEED Role

"B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• propanoyl CoA degradation I (2/3 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (1226 amino acids)

>LRK54_RS15310 methylmalonyl-CoA mutase family protein (Rhodanobacter denitrificans FW104-10B01)
MSSPAKHVPASATQADTTPLRFVTAASLFDGHDAAINIMRRIIQSQGAEVIHLGHNRSVE
DVVRAALQEDADAIALSSYQGGHVEYFKYMVDMLRERGAGHVRVFGGGGGTITPEEIREL
EAYGVERIYHPNDGMHMGLVHMIEDVVQRAGSAAAKRAGSESHTLPKVDIDDEISIGHML
SAIEEGKLAGAELEHQRKQWKLAGQHTPVLGLTGTGGAGKSSVVDELLLRFLHAFPGMRI
AVLAVDPTRRRSGGALLGDRIRMNSLRSKRVYMRSMATRRQHAATSIVLHDCIDFLKSQP
YDLVIVETAGIGQSDSEIVDLVDFPVYVMTSDYGAASQLEKIDMIDFAELVVLNKFDKRG
AEDALRDVRKQWKRNHTAFKVADEDVPVYPTIASQFNDPGVTWMFANLCRLMREKLHLEP
SPSRGGQGGVALAVPCDWKPELDTTLKEPRATVLIPGNRVRYLAEIAEQGRAINRSIETQ
AEYASKAQHYYEALKELGDPKLPRPLDLYASADLHQPVTTPNASTPQTVIPAQAGIASPT
AHEAHSAKAANSATKSDSGLRRNDGKKEPERDEHAVDRSILLLRQRYNAALKELSHEAIH
QLREWPALYASVTADVNEYKVRDKVIRVDNYRESLSHQKVPKVAPPKSKDWGELVSFLGR
ENLPGHYPYTGGVYPYRRSGEDPTRMFAGEGTPERTNRRFHYLSQGGAATRLSTAFDSVT
LYGEDPAPRPDIYGKIGNSGVNIATLDDMKKLYSGFDLSAPSTSVSMTINGPAPMILAMF
MNTAIDQNVEKYLKQDEARWGAAQRYIDKLYANVPRPQYHGELPKGNDGLGLGLLGVCGD
ELVDVETYARIKADTLTKVRGTVQADILKEDQAQNTCIFSTEFALRMMGDIQQYFVDHKV
RNFYSVSISGYHIAEAGANPISQLAFTLSNGFTIVEYYLARGMKVDDFAPNLSFFFSNGM
DPEYTVIGRVARRIWARAMRERYGASARSQMMKYHIQTSGRSLHAQEIQFNDIRTTLQAM
YALFDNCNSLHTNAYDEAITTPTEESVRRAVAIQMIINKELGLNFNENPWQGSYVVDTLT
DLVEEAVYKEFEAISERGGVLGAMDTMYQRGKIQEESMYYEHKKHDGSLPLIGVNTFLPK
DHGGEIATEIELIRSTEEEKGVQIANVKRYGEARNALASDSLKVLQGTARDRRNVFEQLI
EAVKYNSLGQISHALYEVGGEYRRNM