Protein Info for LRK54_RS14600 in Rhodanobacter denitrificans FW104-10B01

Annotation: bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 728 PF02926: THUMP" amino acids 34 to 153 (120 residues), 45.3 bits, see alignment E=2.5e-15 PF01170: UPF0020" amino acids 162 to 376 (215 residues), 163.9 bits, see alignment E=9.7e-52 PF10672: Methyltrans_SAM" amino acids 510 to 661 (152 residues), 72 bits, see alignment E=1.2e-23

Best Hits

Swiss-Prot: 49% identical to RLMKL_ALLVD: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 49% identity to alv:Alvin_2173)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (728 amino acids)

>LRK54_RS14600 bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL (Rhodanobacter denitrificans FW104-10B01)
MSHYFATCPKGMEYLLRDELVALGAPDVREALAGAHFSGTLETAYKACLWSRLASRILLP
LAEFDAADDDALYRGVQTIDWSEHLAAHATFAVDAGTALSKLTHSQFIGLRTKDAVVDQF
RQRDGSRPGIDTDEPDIRINVRVRRDRATVSLDLAGSPLHRRGWREEQGEAPLKENLACA
MLLRARWPEVYAAGGALLDPMCGSGTLLIEGALMAADVAPGLRREYFGFLGWQQHDIALW
RGLLDEAKQRAETGLRGLRSVFFGSDADPRMVQTAKRNAQAAGVAGFFTLDRQDVVHAVP
PPGVDHGLVITNPPYGERLGDRAEMPKLYRALGDTLRQHFIGWRAAVLAGDVELGRAMQL
HADKRYALYNGALETVLLTFDLKPRDEIPRELKPLSAGAQMLKNRLEKNVRHLRKRLLRE
DIHCWRAYDQDLPEYAAAIDVYGDVHGDDHLHIQEYRAPADIPADVARLRLREIARVAGE
VLGVPRERIAIKTRERGKGGSKYGQLDQRGEFIEVEEGGLKFLVNLTDYLDTGLFLDHRL
VRAKVRELAEGRCFLNLFSYTATASVYAAAGGALETTSVDLSATYLEWASRNLALNGFSG
VRHRLMQFDALEFLQRDRGHYGLIFVDPPTFSNSKRADDFDVQRDHVALLEACNGRLTRD
GVIVFSNNFRRFKLDREALEAHFDIEDWSAPSIPFDFARRADIHGCWLLRRRQAVTGINP
WDTAHIKK