Protein Info for LRK54_RS12375 in Rhodanobacter denitrificans FW104-10B01

Annotation: leucyl aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF02789: Peptidase_M17_N" amino acids 22 to 144 (123 residues), 102.3 bits, see alignment E=1.9e-33 PF00883: Peptidase_M17" amino acids 184 to 486 (303 residues), 416.8 bits, see alignment E=6e-129

Best Hits

Swiss-Prot: 59% identical to AMPA_XYLFT: Probable cytosol aminopeptidase (pepA) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 62% identity to psu:Psesu_2427)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (496 amino acids)

>LRK54_RS12375 leucyl aminopeptidase (Rhodanobacter denitrificans FW104-10B01)
MTLQFSLGSAAPATVDSACVLVGVYEQGVLTSAAAQLDSAAGGMIKRQVESGDISGKAGS
TTVLFAPAGVAAQRVLVVGLGAQKSFDAARYQKVNIEAARSLGRLPVANAVSYLSEVEVP
GRDSAWRVRIAALACDHAAYRYTATFKPRDKSKQTELAALTFAAGTDAQGGLDQAVAIAE
GVRFARELANLPPNICNPAYIAAQAQAFAEAQGKVSCSVLDEAAMAKLGFGSLLAVARGS
VNKPRLIALEYKGGHDGAKPYVLVGKGVTFDSGGISLKPGAGMEEMKFDMGGAAGVLGAF
VAAVKMGLKLNLACVVPAVENMPDGDSYRPSDVLTSLSGLTIEVLNTDAEGRLILCDALT
YAAQTYQPQALVDAATLTGACVIALGKHATGLMSKHDDLAAELLAAGEETLDRAWRLPLW
DDYQAQLESGFADVANIGGKSAGAITAGCFLSRFTDGQRWAHLDIAGTAWEEGRKGLATG
RPVALLAQWLLDRSEQ