Protein Info for LRK54_RS12190 in Rhodanobacter denitrificans FW104-10B01

Annotation: 16S rRNA (cytidine(1402)-2'-O)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 274 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR00096: 16S rRNA (cytidine(1402)-2'-O)-methyltransferase" amino acids 8 to 273 (266 residues), 260.3 bits, see alignment E=1.1e-81 PF00590: TP_methylase" amino acids 8 to 208 (201 residues), 110.9 bits, see alignment E=4.3e-36

Best Hits

Swiss-Prot: 62% identical to RSMI_XYLFT: Ribosomal RNA small subunit methyltransferase I (rsmI) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K07056, (no description) (inferred from 67% identity to xal:XALc_2824)

MetaCyc: 52% identical to 16S rRNA 2'-O-ribose C1402 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11637 [EC: 2.1.1.198]

Predicted SEED Role

"rRNA small subunit methyltransferase I" in subsystem Heat shock dnaK gene cluster extended

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.198

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (274 amino acids)

>LRK54_RS12190 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (Rhodanobacter denitrificans FW104-10B01)
MSAPLGCLWVVATPIGHRDDLSARAIETLRAVAVIAAEDTRHSRPLLLHHNIDTPLIALH
EHNERDAVEAIVRRMQGGEAVALISDAGTPLISDPGFRLVRAARAAGIRCIPVPGACAAI
AALSVAGLPSDRFVFEGFLPPKAAARRSRLQELAGDARTLIFYESSHRVAESLADMRDVF
GAGREAVLARELTKLFETVIGEPLAELAERVANDPDQQRGECVILVAGRGEEADAKLAEG
RRVFAILREELPPAKAAKLAAAISGAPRRALYES