Protein Info for LRK54_RS12020 in Rhodanobacter denitrificans FW104-10B01

Annotation: branched-chain amino acid transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 58% identical to ILVE_ECOL6: Branched-chain-amino-acid aminotransferase (ilvE) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00826, branched-chain amino acid aminotransferase [EC: 2.6.1.42] (inferred from 62% identity to avr:B565_3839)

MetaCyc: 58% identical to branched-chain-amino-acid aminotransferase (Escherichia coli K-12 substr. MG1655)
Branched-chain-amino-acid transaminase. [EC: 2.6.1.42, 2.6.1.6]; 2.6.1.42 [EC: 2.6.1.42, 2.6.1.6]; 2.6.1.42 [EC: 2.6.1.42, 2.6.1.6]; Aspartate transaminase. [EC: 2.6.1.42, 2.6.1.6, 2.6.1.1, 2.6.1.27, 2.6.1.57]

Predicted SEED Role

"Branched-chain amino acid aminotransferase (EC 2.6.1.42)" in subsystem Alanine biosynthesis or Branched-Chain Amino Acid Biosynthesis or Isoleucine degradation or Leucine Biosynthesis or Leucine Degradation and HMG-CoA Metabolism or Pyruvate Alanine Serine Interconversions or Valine degradation (EC 2.6.1.42)

MetaCyc Pathways

superpathway of aromatic amino acid biosynthesis (18/18 steps found)
aspartate superpathway (23/25 steps found)
superpathway of branched chain amino acid biosynthesis (17/17 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
superpathway of L-phenylalanine biosynthesis (10/10 steps found)
superpathway of L-tyrosine biosynthesis (10/10 steps found)
superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
anaerobic energy metabolism (invertebrates, cytosol) (7/7 steps found)
L-leucine biosynthesis (6/6 steps found)
superpathway of L-threonine biosynthesis (6/6 steps found)
L-valine biosynthesis (4/4 steps found)
superpathway of L-alanine biosynthesis (4/4 steps found)
L-asparagine degradation III (mammalian) (3/3 steps found)
L-phenylalanine biosynthesis I (3/3 steps found)
L-tyrosine biosynthesis I (3/3 steps found)
superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
L-leucine degradation I (5/6 steps found)
TCA cycle VIII (Chlamydia) (5/6 steps found)
L-alanine biosynthesis I (2/2 steps found)
L-phenylalanine biosynthesis III (cytosolic, plants) (2/2 steps found)
malate/L-aspartate shuttle pathway (2/2 steps found)
superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
L-tyrosine degradation I (4/5 steps found)
3-(4-hydroxyphenyl)pyruvate biosynthesis (1/1 steps found)
L-aspartate biosynthesis (1/1 steps found)
L-aspartate degradation I (1/1 steps found)
L-isoleucine biosynthesis II (6/8 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
superpathway of L-threonine metabolism (13/18 steps found)
L-isoleucine biosynthesis V (2/3 steps found)
L-isoleucine degradation II (2/3 steps found)
L-leucine degradation III (2/3 steps found)
L-valine degradation II (2/3 steps found)
L-isoleucine biosynthesis IV (4/6 steps found)
L-isoleucine degradation I (4/6 steps found)
anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
L-glutamate degradation II (1/2 steps found)
L-tryptophan degradation IV (via indole-3-lactate) (1/2 steps found)
L-tyrosine degradation II (1/2 steps found)
atromentin biosynthesis (1/2 steps found)
L-phenylalanine degradation III (2/4 steps found)
L-tyrosine degradation III (2/4 steps found)
C4 photosynthetic carbon assimilation cycle, NAD-ME type (7/11 steps found)
L-isoleucine biosynthesis III (4/7 steps found)
(R)-cysteate degradation (1/3 steps found)
L-isoleucine degradation III (oxidative Stickland reaction) (1/3 steps found)
L-leucine degradation V (oxidative Stickland reaction) (1/3 steps found)
L-phenylalanine degradation II (anaerobic) (1/3 steps found)
L-tyrosine degradation IV (to 4-methylphenol) (1/3 steps found)
L-valine degradation III (oxidative Stickland reaction) (1/3 steps found)
indole-3-acetate biosynthesis VI (bacteria) (1/3 steps found)
sulfolactate degradation III (1/3 steps found)
superpathway of plastoquinol biosynthesis (2/5 steps found)
L-tryptophan degradation VIII (to tryptophol) (1/4 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
superpathway of chorismate metabolism (40/59 steps found)
superpathway of sulfolactate degradation (2/6 steps found)
4-hydroxybenzoate biosynthesis I (eukaryotes) (1/5 steps found)
L-leucine degradation IV (reductive Stickland reaction) (1/5 steps found)
L-phenylalanine degradation VI (reductive Stickland reaction) (1/5 steps found)
L-tryptophan degradation XIII (reductive Stickland reaction) (1/5 steps found)
L-tyrosine degradation V (reductive Stickland reaction) (1/5 steps found)
L-valine degradation I (3/8 steps found)
coenzyme M biosynthesis II (1/6 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (2/9 steps found)
indole-3-acetate biosynthesis II (3/12 steps found)
tropane alkaloids biosynthesis (2/11 steps found)
rosmarinic acid biosynthesis I (1/10 steps found)
(S)-reticuline biosynthesis I (1/11 steps found)
superpathway of hyoscyamine (atropine) and scopolamine biosynthesis (4/16 steps found)
superpathway of rosmarinic acid biosynthesis (1/14 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)
anaerobic aromatic compound degradation (Thauera aromatica) (1/27 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.1, 2.6.1.42, 2.6.1.57

Use Curated BLAST to search for 2.6.1.1 or 2.6.1.27 or 2.6.1.42 or 2.6.1.57 or 2.6.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (305 amino acids)

>LRK54_RS12020 branched-chain amino acid transaminase (Rhodanobacter denitrificans FW104-10B01)
MAQQYPEWIWQNGSIKPWAEATTHVMSHALHYGSSVFEGIRSYVTPDGAAIFRLTDHLNR
LYMSAKIYDMALPYTVDELAAACREVIRRNGLGASYLRPVAWRGLGGFGLSAETPIDMAV
AAWPMGPYLGAEALQNGIDACVSSWQRFAPNTIPAGAKAGGNYLSGQLIAREARRLGFGE
GIALASTGLLSEGAGENLFLVFDGVLHTPPASASLLTGITRNTLMTLAREDGIEVVERDI
PREYLYLAEEVLMCGTAAEITPIRSIDGKQVGTGKAGRVTLRLQELFFGLFDGRTHDKWG
WLEQV