Protein Info for LRK54_RS10590 in Rhodanobacter denitrificans FW104-10B01

Annotation: malate synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 22 to 71 (50 residues), 63.1 bits, 2e-21
• TIGR01344: malate synthase A
  • amino acids 25 to 541 (517 residues), 721.4 bits, E=2.2e-221
• PF01274: MS_TIM-barrel
  • amino acids 163 to 407 (245 residues), 350 bits, E=7.9e-109
• PF20659: MS_C
  • amino acids 413 to 541 (129 residues), 93.1 bits, E=2.6e-30

Best Hits

Swiss-Prot: 63% identical to MASY_MYXXD: Malate synthase (mls) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 66% identity to psu:Psesu_0138)

MetaCyc: 56% identical to malate synthase A (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glycol metabolism and degradation (4/7 steps found)
• L-arabinose degradation IV (2/8 steps found)
• D-xylose degradation IV (1/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)
• superpathway of pentose and pentitol degradation (4/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Protein Sequence (541 amino acids)

>LRK54_RS10590 malate synthase A (Rhodanobacter denitrificans FW104-10B01)
MAVPQERLDTGSVEIHADPAGYAGILTPAALAFLARLHRRFEATRRLLLQARRERQARYD
AGDLPDFRADTAAIRDGDWRVAPIPAALQDRRVEITGPVERKMIINALNSGAKVFMADFE
DSSSPTFTNQLDGQLNLRDAVNGTIAFTSPEGREYRVNDKPAVLVVRPRGWHLHDRFFSV
DGEAVAGALVDFGLFAFHNARALHARDRGPYFYLPKLEAMEEAALWDAVMAAAEDELQLP
PGTMKATVLIETLPAAFQMHEILHALRRRAVGLNCGRWDYIFSYLKTLRGHRDRLLPERG
QVQMTAPFLKAYSELLIQTCHRRGAFAMGGMAAQIPIKGDEAANEAALAKVRADKLREVK
AGHDGTWVAHPALVPVAQQIFDEYMPAPNQLGVLREDVQVSREQLLAAPAGTITRAGFDN
NVEVCLRYTAAWLDGLGCVPIHHLMEDAATAEIARAQLWQWLHHPDVPAGRAPAGGALEF
PDHAPIDFALFDHAVAVHTHRLRDSRHPGAARADAAAALLSAMTHADRLGDFLTLPAYEQ
L