Protein Info for LRK54_RS10475 in Rhodanobacter denitrificans FW104-10B01

Annotation: type I restriction-modification system subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 transmembrane" amino acids 376 to 399 (24 residues), see Phobius details PF12161: HsdM_N" amino acids 9 to 136 (128 residues), 73.7 bits, see alignment E=3.7e-24 PF02384: N6_Mtase" amino acids 156 to 435 (280 residues), 235 bits, see alignment E=2.1e-73 PF01170: UPF0020" amino acids 200 to 289 (90 residues), 24.5 bits, see alignment E=3.1e-09

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 70% identity to slt:Slit_2437)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (691 amino acids)

>LRK54_RS10475 type I restriction-modification system subunit M (Rhodanobacter denitrificans FW104-10B01)
MTKEQLKKLEADLWRSADSLRANSDLKASEYSTPVLGLIFLKFADNNYRKHEAAILAEYG
KHKGKRAERELAAIAIEQCGFYLPEHARYDYLLNLPDDQDIARAIKLAMQAIEQYKPELA
GVLPQDEYFRLTRSEQNRGIPRQLLKNFADIPADADGDMFGQIYEYFLGEFAMSEGQGGG
EFFTPRSVVRLMVGIIQPHGGTMYDPACGSGGMFVQSAHFIEQQRRDTAEGGADIYVYGQ
EKTLETVKLAKMNLAVNGLRGEIKQANTYYEDPHDSFGAFDYVLANPPFNVDDVSLGSVE
GDRRFNTYGIPRNKTKVKKADEGKQTVPNGNYLWINLFATSLNERGRAALVMANSASDAR
HSEADIRKALIEANLIYGMLTLPSNMFYTVTLPATLWFFDKAKDDDRILFIDARNVFTQI
DRAHREFSDEQVRNLALISQLHLGDRHEFIEAVDDYFAAGFAALAESHAQIAPLAEQLLA
VLDDAEGQQAVAAMQAEWAALKPLLARYAKYRAKAEASASTEASNRAQLGLREHCDPFFV
ALHADLKQLDKRVRAHEKKLADAAQAEGRRGGTGRDIRALKVALDALHAEVRRAEYWFGH
VRWLQERFPNAEYEDVTGLCKLATPAEVAEQEYSLNPGRYAGVVIEEDGKTEEEFVAELL
AMNEEFAKLNEDARTLEDLISSNLKSIVDMA