Protein Info for LRK54_RS10245 in Rhodanobacter denitrificans FW104-10B01
Annotation: pimeloyl-ACP methyl ester esterase BioH
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to BIOH_STRM5: Pimeloyl-[acyl-carrier protein] methyl ester esterase (bioH) from Stenotrophomonas maltophilia (strain R551-3)
KEGG orthology group: K02170, biotin biosynthesis protein BioH (inferred from 54% identity to smt:Smal_3829)MetaCyc: 35% identical to pimeloyl-acyl carrier protein methyl ester esterase (Escherichia coli K-12 substr. MG1655)
RXN-11483 [EC: 3.1.1.85]; Carboxylesterase. [EC: 3.1.1.85, 3.1.1.1]
Predicted SEED Role
"Biotin synthesis protein BioH"
MetaCyc Pathways
- biotin biosynthesis I (14/15 steps found)
- 8-amino-7-oxononanoate biosynthesis I (10/11 steps found)
- methylsalicylate degradation (2/2 steps found)
- aspartame degradation (1/2 steps found)
- methyl indole-3-acetate interconversion (1/2 steps found)
- superpathway of methylsalicylate metabolism (1/5 steps found)
- retinol biosynthesis (1/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.1
Use Curated BLAST to search for 3.1.1.1 or 3.1.1.85
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (261 amino acids)
>LRK54_RS10245 pimeloyl-ACP methyl ester esterase BioH (Rhodanobacter denitrificans FW104-10B01) MSLYVDRRGHGPVPLVLLHGWAMHGGVLTPLVEALENQCTIYVVDLPGHGRSRDCGLPLE PHACAAAIAKLTPPALWLGWSLGGTIALTAALELPRQVRGLAMLCATPKFVRDAGWPHGS DAQLVHQLASDLETDYHATIERFLALEAMGSADPRGELRHLRELVFTHGEPDLRVLQEGI RILESSDRRAALPDLAVRSAWISGRRDRLVPPQAMAWSASQCGGQYTEIPQAGHAPFFGH ADAVAQALQPLLAPLSLEQAR