Protein Info for LRK54_RS10240 in Rhodanobacter denitrificans FW104-10B01

Annotation: malonyl-ACP O-methyltransferase BioC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 TIGR02072: malonyl-acyl carrier protein O-methyltransferase BioC" amino acids 14 to 263 (250 residues), 221.1 bits, see alignment E=9.6e-70 PF13489: Methyltransf_23" amino acids 30 to 190 (161 residues), 53.6 bits, see alignment E=7.6e-18 PF00891: Methyltransf_2" amino acids 36 to 143 (108 residues), 25.3 bits, see alignment E=3.1e-09 PF01209: Ubie_methyltran" amino acids 46 to 173 (128 residues), 35.6 bits, see alignment E=2.3e-12 PF13847: Methyltransf_31" amino acids 46 to 162 (117 residues), 43.8 bits, see alignment E=8.1e-15 PF13649: Methyltransf_25" amino acids 50 to 143 (94 residues), 71.5 bits, see alignment E=2.8e-23 PF08241: Methyltransf_11" amino acids 51 to 147 (97 residues), 83.9 bits, see alignment E=3.5e-27 PF08242: Methyltransf_12" amino acids 51 to 144 (94 residues), 50.4 bits, see alignment E=1.1e-16

Best Hits

Swiss-Prot: 45% identical to BIOC_XYLF2: Malonyl-[acyl-carrier protein] O-methyltransferase (bioC) from Xylella fastidiosa (strain M23)

KEGG orthology group: K02169, biotin synthesis protein BioC (inferred from 53% identity to smt:Smal_3831)

Predicted SEED Role

"Biotin synthesis protein BioC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (292 amino acids)

>LRK54_RS10240 malonyl-ACP O-methyltransferase BioC (Rhodanobacter denitrificans FW104-10B01)
MSDFQLDPTQVCRHFGRAASTYEKHDALQREVGDALLERLGFYLETPLRVVDVGAGTGRG
SALLKQKYPKAEVIAVDLALPMLRAAKQHSSWRKPFRRVCAEATRLPLADHSVDVLYSNL
CFQWVDDLPALFGECMRVLKPGGFMAFSSFGPDTLMELRAAWAEADQQPHVGRFLDMHDV
GDAMLNAGLRDPVLDVFRYTLTYSEPRKLLEELRGLGATNADRARARGLTGKGRYRRMLA
AYEAMRTDGRIPASWEVVSAHAWGPPVGQPRRVPGGEIATFSIDTLRGSRRK