Protein Info for LRK54_RS10200 in Rhodanobacter denitrificans FW104-10B01

Annotation: aminopeptidase N

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 884 TIGR02414: aminopeptidase N" amino acids 17 to 880 (864 residues), 1071.9 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 103 to 197 (95 residues), 35.3 bits, see alignment E=2.7e-12 PF01433: Peptidase_M1" amino acids 236 to 445 (210 residues), 179 bits, see alignment E=2.2e-56 PF11940: DUF3458" amino acids 455 to 559 (105 residues), 92.4 bits, see alignment E=4e-30 PF17432: DUF3458_C" amino acids 565 to 882 (318 residues), 324.6 bits, see alignment E=1.6e-100

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 48% identity to gbe:GbCGDNIH1_0675)

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.2

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (884 amino acids)

>LRK54_RS10200 aminopeptidase N (Rhodanobacter denitrificans FW104-10B01)
MHALSTQAASASICLRDYRAPAWRVDTVELDFDLGIDASEVLARLHLRRDPAQDAPLWLD
GENLQLLSIALDGTPLPATAYRYDGNALEVDGARDGSVLETRVRLRPAANTALEGLYLSG
PRETGFLLTQCEAEGFRHITFFPDRPDVLSRYTVTLRADRTRFPVLLAGGNPDGAGALDG
GRHWARFVDPHPKPSYLFAVVAGHLQVMERDYVTADDHAVQLKIWAEADAIDRCHYAMDA
LERAMRWDEQAYGRNYDLDVFHVVATHDFNMGAMENKGLNIFNAKCLLAAPDSTTDEEYR
HVEAVVAHEYFHNWSGNRVTCRDWFQLSLKEGLTVFREQSFSADMNSAPLKRIEDVALLR
RAQFPEDAGPLAHPVRPAQYSEINNFYTATVYEKGAELVRMLAGALGRDGFRRGMDRYFE
RNDGRAATLEDFLAALGGANGTDLTPYLAWYAQAGTPRLKAHGHYDAVRHTYTLTLAQYT
PAGHGAEPRVPLPIPVKLALFTPQGDMLPLHLDGQTAKPDTERVVVLERAEQSFVFADVT
TAPVPSLLRGFSAPVILECDYTPGELALLLAHDVDGFNRWEAGQQLAARAYEDLRDDTAG
AALHAWCDALARLFDAAAIDDALLADLLTPPGEVELAERESAIDPQRIHHLRQALQQRLA
ARLGAPALRQRYAALAAHTTIELDAASQAQRRLKRRVLELLALLDPAGAGELATAQYREA
PGMTDRLAALSVLVRSDAPQAAAALRDYRERHAGNALALDKWFAVQAQLPGERALDRVRR
LERDAAFTLKNPNRVQSLLGAFVRNNPSGFHRVDGAGYRLLAERLVALDALNPQVAARLA
TAFNGWQRLEPLRREAAHAAIVELAGHHGLSRNLAEIIGSVLNR