Protein Info for LRK54_RS09630 in Rhodanobacter denitrificans FW104-10B01

Annotation: mercury(II) reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 PF00403: HMA" amino acids 12 to 56 (45 residues), 45.7 bits, see alignment 3.1e-15 PF07992: Pyr_redox_2" amino acids 98 to 416 (319 residues), 210.3 bits, see alignment E=1.9e-65 TIGR02053: mercury(II) reductase" amino acids 98 to 559 (462 residues), 591.6 bits, see alignment E=6.2e-182 PF01134: GIDA" amino acids 99 to 240 (142 residues), 26.3 bits, see alignment E=1.8e-09 PF00890: FAD_binding_2" amino acids 99 to 133 (35 residues), 30.1 bits, see alignment 1.4e-10 PF00070: Pyr_redox" amino acids 270 to 338 (69 residues), 43.7 bits, see alignment E=1.4e-14 PF02852: Pyr_redox_dim" amino acids 435 to 543 (109 residues), 104.5 bits, see alignment E=1.7e-33

Best Hits

Swiss-Prot: 66% identical to MERA_ACIFR: Mercuric reductase (merA) from Acidithiobacillus ferrooxidans

KEGG orthology group: K00520, mercuric reductase [EC: 1.16.1.1] (inferred from 66% identity to afr:AFE_2481)

Predicted SEED Role

"Mercuric ion reductase (EC 1.16.1.1)" in subsystem Mercuric reductase or Mercury resistance operon (EC 1.16.1.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.16.1.1

Use Curated BLAST to search for 1.16.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (559 amino acids)

>LRK54_RS09630 mercury(II) reductase (Rhodanobacter denitrificans FW104-10B01)
MTETYDLNKQALEVSGMTCQACARHVQEALSSVDGVTSASVDYPTATAQVVAGAALSVAA
LNAALPPPYRVRALARPPAADHAARHGGPVAGHDARLRVAVIGTGGAAMAAALRAAERGA
RVTVIERGTVGGTCVNIGCVPSKIMIRAAHVAHTRRHSPFDGGIDAGAPAIRRDALLAQQ
QARVEELRHAKYERILDGNPDITLLRGEARFVDGHTLGVRLAEGGERTLAFDRCLIAVGA
RPAVPPIPGLAGTPYWTSTDALAADHIPRRLLVLGSAVVAAELGQAYARLGSDVTVIARS
ELFFREDPAVGEAIGRIFADEGIRVLRQTQTRAVRHENGEFILDTSAGQLRGDALLVATG
RTPNGDTLELAQAGVRTGKGGAIEVDAQLRTSVAHIYAAGDCTDQPQFVYVAAAGGTRAA
INMTGGDAQLDLSAMPAVVFTEPQIATVGLSEAEAHLKGIETDSRTLTLDNVPRALANFD
TRGFIKLVVEAASGRLIGVQAVADGAGELIQTAAIALRAGMTAAELADQLFPYLTMVEGL
KLAAQTFSKDVKQLSCCAG