Protein Info for LRK54_RS09135 in Rhodanobacter denitrificans FW104-10B01

Annotation: polyamine aminopropyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 TIGR00417: spermidine synthase" amino acids 12 to 282 (271 residues), 267.3 bits, see alignment E=7e-84 PF17284: Spermine_synt_N" amino acids 12 to 63 (52 residues), 45.7 bits, see alignment 5e-16 PF01564: Spermine_synth" amino acids 68 to 246 (179 residues), 176.4 bits, see alignment E=4.5e-56

Best Hits

Swiss-Prot: 67% identical to SPEE_METCA: Polyamine aminopropyltransferase (speE) from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

KEGG orthology group: K00797, spermidine synthase [EC: 2.5.1.16] (inferred from 65% identity to psu:Psesu_0148)

Predicted SEED Role

"Spermidine synthase (EC 2.5.1.16)" in subsystem Polyamine Metabolism (EC 2.5.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>LRK54_RS09135 polyamine aminopropyltransferase (Rhodanobacter denitrificans FW104-10B01)
MSQQPTSNETSWFTEAHQASGSSIGFRTEQLLHAEKTPFQTIEIHQTTDWGKLMVIDGCV
MLTSRDNFLYHEMMTHPALFTHARAKRVVIIGGGDCGTLREVLKHEEVEHAVQVEIDERV
TRLAEQYFPELCEANGDPRAELLFIDGIKYMAEAEPDSLDLIIVDSTDPVGPAEGLFNAA
FYASCYKALRHGGLLVQQSESPLAHLELIKSMRSAMRTSGFSAVKTLPFPQPCYPTGWWS
CTMARKGGDLSGFRERGASAKNFPTRYYNAEIHKAALAQPEFMREALGD