Protein Info for LRK54_RS08080 in Rhodanobacter denitrificans FW104-10B01

Annotation: GMC family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 547 PF01266: DAO" amino acids 8 to 42 (35 residues), 32.6 bits, see alignment 1.6e-11 PF13450: NAD_binding_8" amino acids 11 to 41 (31 residues), 27.2 bits, see alignment (E = 9.9e-10) PF00732: GMC_oxred_N" amino acids 215 to 314 (100 residues), 27.6 bits, see alignment E=6.4e-10 PF05199: GMC_oxred_C" amino acids 402 to 522 (121 residues), 78.2 bits, see alignment E=2.4e-25

Best Hits

KEGG orthology group: None (inferred from 69% identity to har:HEAR2557)

Predicted SEED Role

"2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.4)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.4

Use Curated BLAST to search for 1.1.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (547 amino acids)

>LRK54_RS08080 GMC family oxidoreductase (Rhodanobacter denitrificans FW104-10B01)
MSGQLSADIVVIGSGIIGSLAARRLAQQGASVLILEAGPRLERARVVAAFRNSPIKGNWM
APYPPSPWAPHPIYVPEDNGYLKQAGPYPYPAEYIRAVGGTTWHWAAQAWRLVPNDVRIK
SLYGVGVDWPLTYEELDPWYQEAEEILGVAGADNTGSPRQHPFPMEPVAEPWSMRRFRER
LAPVYPVVANTVARNSRGYDGRPACVGNNSCQPICPVNAQYLGINAVEAAEAAGAKVVPN
AVVYRIEHDAKGRIAAVHYYAPDKSEHRVTGATFILAANGIESPKLLLLSASDKYPNGLA
NSSGMVGRHLMDHPSTSLTFYADEELWLGRGPQSPSSINTLRDGAFRSQHAPYRLDFTNI
SQVLAVTNDLIKEGVYGAEFAKQLRWRAARQVNVKNVLEVLPNPDNRITLSAEKDAMGIP
KPEAHYAIDDYTRRAAEVSKADFARIAELMGGTGLRYSAEGNFANNQHITGTMSMGNDPS
SSVVDAFGRTHDHENLFICSTGVMPTAATMNSTLTGVALALRTAEHILPAKASRTTTADS
GREAGLA