Protein Info for LRK54_RS07880 in Rhodanobacter denitrificans FW104-10B01

Annotation: phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 TIGR01196: phosphogluconate dehydratase" amino acids 5 to 604 (600 residues), 968.9 bits, see alignment E=4.4e-296 PF00920: ILVD_EDD" amino acids 68 to 602 (535 residues), 504.6 bits, see alignment E=1.6e-155

Best Hits

Swiss-Prot: 62% identical to EDD_RHIME: Phosphogluconate dehydratase (edd) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 67% identity to nda:Ndas_4741)

MetaCyc: 58% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (605 amino acids)

>LRK54_RS07880 phosphogluconate dehydratase (Rhodanobacter denitrificans FW104-10B01)
MTTLHPVVAEVTERLRQRSRDTRAAYLRRIDAVDRSGPQREHLSCGNLAHGFAACGSEDK
AALRSGRRANLAIVTAYNDMLSAHQPYERYPALIRQVARDAGFTAQVAGGVPAMCDGITQ
GRPGMQLSLFSRDVVALATAVALSHDMFDGALFLGICDKIVPGLLIGALSFGHLAGAFVP
SGPMPSGIPNEQKSKVRQAFADGKASKAELLEAEAASYHAPGTCTFYGTANSNQMLMEIM
GLHLPGASFTAPDTPLRDALTAEVVRRVAAHSALGDASLPLGHIIDERAIINGVIGLHAT
GGSTNHLLHLVAIAHAAGIQLRWDDFDALSGVIPLLARVYPNGYADVNQFHDAGGMAFLI
DQLLGAGLLHADVQTIFGTGLDGYAQVPALDAGGALHWQPVAKQSGNRGVLRGVDEPFRP
DGGLRMLDGNLGRAVIKVSSVPDDRLLIEAPAIVFDDQDEVAGAFKRGQLNRDFVAVVRF
QGPRANGMPELHKLTPTLALLQDRGHRIALLTDGRMSGASGRVPAAIHVTPEAVAGGNLA
KIRDGDMIRLDAANGRLDVLLEAHELDARLPHVADLSGEHSGMGRELFGLFRQAAANADL
GAGVL