Protein Info for LRK54_RS06535 in Rhodanobacter denitrificans FW104-10B01

Annotation: glycerol kinase GlpK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 PF00370: FGGY_N" amino acids 5 to 252 (248 residues), 260.2 bits, see alignment E=2.1e-81 TIGR01311: glycerol kinase" amino acids 5 to 494 (490 residues), 713.8 bits, see alignment E=4.3e-219 PF02782: FGGY_C" amino acids 262 to 450 (189 residues), 149.5 bits, see alignment E=1.1e-47

Best Hits

Swiss-Prot: 67% identical to GLPK_GEOSW: Glycerol kinase (glpK) from Geobacillus sp. (strain WCH70)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 67% identity to gwc:GWCH70_1268)

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (499 amino acids)

>LRK54_RS06535 glycerol kinase GlpK (Rhodanobacter denitrificans FW104-10B01)
MAQRYVLAIDQGTTSSRAILFDRAGGVVGSAQREFRQIFPRPGWVEHDPQEILASVLATV
GEVLGAARVGARELAGIGIANQRETTVVWDRHTGRPVRNAIVWQSRQSLAICEGLEAEGY
AALVRQKTGLLIDAYFSGSKIRWILDHVAGARARAERGDLLFGTIDSWLLWNLTGGGLHA
TDVSNAARTLLFDIHMRQWDDELLRMLDIPRSMLPEVRSCSEVYGRTVALPGVPAGVPIS
GVAGDQQAALFGQACFEPGMAKNTYGTGCFMLMHTGVRAVPSRHGLLTTIAWELDGAVEY
ALEGSIFVAGSVVQWLRDGLQILARTSDSQACAESVESSEGVYVVPAFVGLGAPYWRSDV
RGAMFGLSRGTRKEHVVRAALESMAYQSRDVLAAMEADASIPLKELRADGGAIANDFMAQ
FQSDMLGVPVLRPKVAETTALGAAYLAGLAVGFWESREQIAALWQVDRRFLPAMEASRRE
RLYRGWLDAVNATIGFRVD