Protein Info for LRK54_RS05995 in Rhodanobacter denitrificans FW104-10B01

Annotation: tRNA dihydrouridine(20/20a) synthase DusA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 TIGR00742: tRNA dihydrouridine synthase A" amino acids 12 to 321 (310 residues), 438 bits, see alignment E=1.1e-135 PF01207: Dus" amino acids 15 to 309 (295 residues), 283.1 bits, see alignment E=1.4e-88

Best Hits

Swiss-Prot: 66% identical to DUSA_XANCP: tRNA-dihydrouridine(20/20a) synthase (dusA) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 66% identity to sml:Smlt0275)

Predicted SEED Role

"tRNA dihydrouridine synthase A"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (328 amino acids)

>LRK54_RS05995 tRNA dihydrouridine(20/20a) synthase DusA (Rhodanobacter denitrificans FW104-10B01)
MAENHHRAPPWQLSVAPMMDWTDRHCRYFHRLLSPRARLYTEMVTSAALVRGKQLRLLEH
SQQEHPLALQLGGSEPYELAQAARLGAEAGYDEINLNVGCPSDRVQSGRFGACLMYEPAL
VADCVKAMRDAVSVPVTVKCRIGVDDQDDYAGLQQFTELMLEAGVEVLVVHARKAWLKGL
SPKENREVPPLDYARVYRLKREFPPLVVVINGGISTVDQVRSHLAQLDGVMLGRAAYHDP
YLLAQLEHALHDEPLPRREDVLQRLRPYVEAELARGTALKHISRHLLGLYQGEPGARAFR
RTLSEGAHLPGAGWALLEQAMAPCAAAA