Protein Info for LRK54_RS05830 in Rhodanobacter denitrificans FW104-10B01

Annotation: helix-turn-helix domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 PF00027: cNMP_binding" amino acids 59 to 139 (81 residues), 51.7 bits, see alignment E=1.1e-17 PF13545: HTH_Crp_2" amino acids 172 to 246 (75 residues), 64.7 bits, see alignment E=8.9e-22 PF00325: Crp" amino acids 197 to 228 (32 residues), 43.4 bits, see alignment 3.3e-15

Best Hits

Swiss-Prot: 40% identical to ANR_PSEPH: Transcriptional activator protein Anr (anr) from Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CHA0)

KEGG orthology group: K01420, CRP/FNR family transcriptional regulator, anaerobic regulatory protein (inferred from 60% identity to psu:Psesu_0364)

Predicted SEED Role

"Fumarate and nitrate reduction regulatory protein" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (253 amino acids)

>LRK54_RS05830 helix-turn-helix domain-containing protein (Rhodanobacter denitrificans FW104-10B01)
MQSKPMAGRLPEPPSPIADDGDETRFCGTCAFSSACIAAGYDKPELAELQCLVEHVGPFR
AGEHIFRTGDPFRAIFAVRAGTVKTRMVDKEGREQVLGFYLPGEVIGLNAIYPEHFPCDA
VALDTAYFCRFSFPAMSALASRIPAVQQHLFRMLSKELGTASLLAGDHSADERVAAFLMD
LAGRYAVRGFSATRFHLSMSRGDIANYLRLAAETVSRVLSRFRSQQLIEIEGRELELLNP
KKLREIGQALLPE