Protein Info for LRK54_RS04955 in Rhodanobacter denitrificans FW104-10B01

Annotation: ATP-dependent chaperone ClpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 860 (855 residues), 1461.5 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 68 (52 residues), 37.9 bits, see alignment 9e-13 amino acids 95 to 145 (51 residues), 43.4 bits, see alignment 1.7e-14 PF00004: AAA" amino acids 203 to 336 (134 residues), 47.6 bits, see alignment E=1.3e-15 amino acids 602 to 727 (126 residues), 37.9 bits, see alignment E=1.3e-12 PF17871: AAA_lid_9" amino acids 342 to 444 (103 residues), 121.1 bits, see alignment E=1e-38 PF07724: AAA_2" amino acids 596 to 765 (170 residues), 238.7 bits, see alignment E=1.9e-74 PF00158: Sigma54_activat" amino acids 596 to 726 (131 residues), 26.1 bits, see alignment E=3.5e-09 PF07728: AAA_5" amino acids 601 to 721 (121 residues), 48.5 bits, see alignment E=5e-16 PF10431: ClpB_D2-small" amino acids 771 to 851 (81 residues), 95.2 bits, see alignment E=1e-30

Best Hits

Swiss-Prot: 71% identical to CLPB_COXBU: Chaperone protein ClpB (clpB) from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 72% identity to aeh:Mlg_2558)

MetaCyc: 76% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (863 amino acids)

>LRK54_RS04955 ATP-dependent chaperone ClpB (Rhodanobacter denitrificans FW104-10B01)
MRMDKLTSRFQQALADAQSLAVGRDHNMLEPVHVMAALLAQQGGSTAPLLTQAQVNVPLL
TQRVNDLLERLPKVSGQEGNINLGNDLNRLLNLTDKLAQQRGDQFIASELFVLAALEDKG
ELGAALKAAGATRANLEAAIEQLRGGEKVQSENAEEQRQALEKYCIDLTARAESGKLDPV
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIIKGEVPEGLRDRRVLAL
DMGALIAGAKFRGEFEERLKAVLSDLSKQEGRVILFIDELHTMVGAGKAEGAMDAGNMLK
PALARGELHCIGATTLDEYRKYIEKDAALERRFQKVFVGEPSVEDTIAILRGLKERYAVH
HGVEITDPAIVAAATLSHRYIADRQLPDKAIDLMDEAASRIRMEIDSKPEELDRLERRLI
QLKIQREMLKKEKDSESKQRLADLETDIGKLEREFSDLNEIWKSEKAALQGTTKVKEQIE
QARQELDAAQRRQDYAKMSEIQYGKLPELEKQLAAAQAAETQDFRLVQTRVTAEEIAEVV
SRWTGIPVNKMLEGERDKLLHMEDALHQRVVGQDEAVHAVSDAIRRSRAGLSDPNRPNGS
FLFLGPTGVGKTELCKALAEFMFDTTDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNL
GSQMIQDAAEGNGDAEEQYTQMKASVLGVVQAHFRPEFINRLDEIVVFRPLDKSQIRAIA
KIQLEYLEKRLAERQLKLDVGDDALALLGNVGFDPVYGARPLKRAIQQQLENPLAKQILE
GRFQSGDTVQVVADGGKLLFRKV