Protein Info for LRK54_RS03685 in Rhodanobacter denitrificans FW104-10B01

Annotation: 50S ribosomal protein L25/general stress protein Ctc

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 PF01386: Ribosomal_L25p" amino acids 7 to 94 (88 residues), 90.1 bits, see alignment E=1e-29 TIGR00731: ribosomal protein bL25, Ctc-form" amino acids 8 to 174 (167 residues), 137.1 bits, see alignment E=2.6e-44 PF14693: Ribosomal_TL5_C" amino acids 103 to 190 (88 residues), 83.5 bits, see alignment E=1.1e-27

Best Hits

Swiss-Prot: 55% identical to RL25_XANAC: 50S ribosomal protein L25 (rplY) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K02897, large subunit ribosomal protein L25 (inferred from 58% identity to smt:Smal_0727)

Predicted SEED Role

"LSU ribosomal protein L25p" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (221 amino acids)

>LRK54_RS03685 50S ribosomal protein L25/general stress protein Ctc (Rhodanobacter denitrificans FW104-10B01)
MSKTHEIKAQSRKDEGKGASRRLRRASFVPAVVYGAGQAPESIQIEHNTILLAAKNEWFF
SSVLDLNVDGKVQKVLVRDWQKHPFKQLMMHMDFLRVDEKAAIRVSVPLHFLNKEKSAAA
KTSGVVISHNLTEVEITCLPKDLPESIELDLADLKPGDIIHLSQLKLPKNVELVALHGGE
GHDTAVVTANTVQEEAEEAPAVEAAAPAPAAAAPAKKDDKK