Protein Info for LRK54_RS03185 in Rhodanobacter denitrificans FW104-10B01

Annotation: phosphoglycerate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 12 to 325 (314 residues), 126.1 bits, E=1.1e-40
• PF02826: 2-Hacid_dh_C
  • amino acids 118 to 293 (176 residues), 174.5 bits, E=2.2e-55
• PF03446: NAD_binding_2
  • amino acids 153 to 253 (101 residues), 21.9 bits, E=2.3e-08

Best Hits

Swiss-Prot: 60% identical to SERA_SHIFL: D-3-phosphoglycerate dehydrogenase (serA) from Shigella flexneri

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 79% identity to xcb:XC_2364)

MetaCyc: 60% identical to phosphoglycerate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Phosphoglycerate dehydrogenase. [EC: 1.1.1.95]; KETOGLUTREDUCT-RXN [EC: 1.1.1.95, 1.1.1.399]; 1.1.1.399 [EC: 1.1.1.95, 1.1.1.399]

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (8/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (3/4 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
• L-serine biosynthesis I (2/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.399 or 1.1.1.95

Protein Sequence (410 amino acids)

>LRK54_RS03185 phosphoglycerate dehydrogenase (Rhodanobacter denitrificans FW104-10B01)
MQTSYPRQDIKVLLLEGVSASAVDNFRRAGYSQVELHAKSLPENELKARIADAHIVGIRS
RTQLTAEVLAQAKRLIAVGCFCIGTNQVDLAAARRLGVPVFNAPYSNTRSVAELVIAEAI
MLLRGIPQKNAQCHRGGWAKSASGSYETRDKVLGIVGYGHIGTQVGVLAESLGMRVIFHD
IETKLALGNARAVSSLDELLERADVVTLHVPETPATRLMIRREQLAKMRAGAMLINASRG
SVVDIDALAAVLRAGHLAGAAVDVFPTEPKGNDDPFVSPLIGLDNVILTPHIGGSTLEAQ
DNIGIEVASKLVRYSDNGSTLSAVNFPEVALPEHPHSRRLLHIHRNVPGTLSRINELFSA
GNINIDAQFLQTDSEVGYVVIDVSADEAQASELKAKLAAIPGTLRSRVLY